Manual

C1000 Touch

Thermal Cycler Manual

• Lid temperature — determines the temperature of the heated lid. If the temperature

is too high, the sample temperature might rise above the target temperature

The C1000 Touch thermal cycler provides two ways of managing and entering sample volume

and lid temperature settings:

• Change the setting when creating and editing the protocol (page 18)

• Change the setting while initiating a run (page 21)

Temperature Control Modes

The C1000 Touch thermal cycler uses one of two temperature control modes to determine

when the sample reaches the target temperature:

• Calculated mode — When a sample volume of greater than zero is entered, the

thermal cycler calculates the sample temperature based on the sample volume. The

calculated mode is recommended, because it most accurately represents the actual

sample temperature

• Block mode — when a sample volume of zero (0) μl is entered, the thermal cycler

assumes that the sample temperature is the same as the measured block

temperature

Choosing Lid Temperature

The adjustable heated lid of the C1000 Touch thermal cycler allows the user to control the lid

temperature and force. Heating the lid prevents condensation from forming inside the reaction

wells. When the C1000 Touch thermal cycler is running, the heated lid maintains the

temperature specified for the protocol being run. Without a heated lid, water can be lost from

the reagents to condensation, concentrating the reactants in the tube or plate.

The default lid temperature of the C1000 Touch thermal cycler is 105°C for 96- or dual 48-well

reaction blocks and 95°C for 384-well blocks.

NOTE: When the block is running an infinite hold at a temperature below 30.0°C,

the lid heater maintains 31.0°C.

Changing Sample Volume

To change the sample volume from the new protocol or protocol editor screen:

1. Touch the Volume button.

2. Enter a value using the pop-up numeric keypad.

3. Touch the OK button to confirm.

NOTE: Entering a value greater than zero in the Volume button selects Calculated

Temperature control mode, which is the standard mode. Entering zero (0) in the

Volume button selects Block mode. Calculated mode is the recommended mode

because it most accurately represents the actual sample temperature.

Changing Lid Temperature

When using a 96- or dual 48-well reaction block, the recommended lid temperature is 105°C.

When using a 384-well block, the recommended lid temperature is 95°C.

To change the lid temperature from the new protocol or protocol editor screen:

1. Touch the Lid button.