Manual

C1000 Touch

Thermal Cycler Manual

• Clean the outer lid surface of the reaction module. Use a damp cloth or tissue to

clean spills off the outside case. If needed, use a mild soap solution and rinse the surface

with a damp cloth. Cleaning the cover prevents corrosion

Maintaining Sufficient Airflow

The C1000 Touch thermal cycler requires sufficient airflow to heat and cool precisely to the

correct target temperature. If the flow of air is blocked, the thermal cycler cannot ramp to the

correct temperature in the specified time. This section includes instructions for testing the

airflow and provides suggestions for fixing low or warm airflow.

Testing for Sufficient Airflow

The airflow is sufficient when the thermal cycler heats and cools to the correct target

temperatures promptly. When the C1000 Touch thermal cycler is first set up in a new location,

use the following steps to determine the presence of sufficient airflow:

1. Set up the instrument in the location where it is going to be used, then turn the power on.

2. Adjust the local environment for typical conditions.

Turn on any nearby equipment, such as fans. Also open any window blinds to reproduce

typical conditions during a run. If more than one thermal cycler is in the area, run a

protocol on all the thermal cyclers at the same time.

3. Run a typical PCR protocol for 30 min.

To run a protocol, samples are not required; however, an empty microplate or tubes

should be included. The lid does not heat correctly if it touches the hot block of the

reaction module.

4. Measure the air temperature at the air intake vents of all the thermal cyclers.

If the air intake temperature increases above 31°C, see “Fixing Insufficient Airflow” to

ensure sufficient airflow.

Fixing Insufficient Airflow

If the air temperature near the thermal cycler is above 31°C, make one or more of the following

changes to increase the flow of cooler air around the thermal cycler:

• Adjust air conditioning to lower the ambient air temperature

• Move the thermal cycler to another location

• Provide more space around the C1000 Touch thermal cycler and between adjacent

instruments. Arrange instruments so that the warm exhaust air from one instrument

does not enter the air intake vents of another

• Shield the thermal cycler from heat sources such as radiators, heat-producing

instruments, and bright sunlight