Software, Version 2.1 Owner's manual

Section 1 Getting Started
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1.5 Calibrating the Instrument
Before using the real-time PCR detection system for the first time, mask alignment, background
calibration, persistent well factor collection, and pure dye calibration (for MyiQ2 and iQ5 systems
only) must be performed. See section 7, Calibrating the Instrument, for detailed instructions on
calibration.
1.6 Compatibility with Earlier Versions of the iQ5 Optical
System Software
Data Files
Protocol, plate setup, and data files generated or saved with version 3.0 or 3.1 of the iQ Real-
Time Detection System software and earlier versions of the iQ5 software are recognized by
version 2.1 of the iQ5 software. These .tmo, .pts, and .opd files can be opened from within iQ5
software for analysis.
To open or import files generated by older versions of the iQ Real-Tme Detection System
software (version 2.3b and earlier), you must open the file in version 3.0 or 3.1 of the iQ
software first and then save the file again.
Calibration Files
Earlier versions of the iQ5 Optical System software (earlier than version 2.1) have volume-
dependent calibration files for both persistent well factors and pure dye calibration collection. In
version 2.1, there is no volume dependency for these calibration files. If a computer is upgraded
from an earlier version of the iQ5 Optical System software to version 2.1, existing calibration files
will be upgraded to remove calibration volume dependency.
For example, Persistent_Plates_Film_25ul_IQ2Emulator_IQ2Emulator.xml will be upgraded
and renamed as Persistent_Plates_Film_IQ2Emulator_IQ2Emulator.xml.
In all cases, the original calibration files with the volume dependency will be backed up to the
backup folder after upgrading.