Software, Version 2.1 Owner's manual

Section 2 Quick Guides

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Editing or Creating a Protocol

Edit the selected protocol by clicking Edit in the Selected Protocol window, or create a protocol

from a protocol template by clicking Create New in the Selected Protocol window.

NOTE: Clicking Edit or Create New in the Selected Protocol pane opens the Protocol Editor.

NOTE: You can only exit the Protocol Editor by clicking Save & Exit Protocol Editing or

Cancel & Exit Protocol Editing.

1. Edit the protocol by performing one or more of the following five tasks:

• Edit the Dwell Time and Setpoint temperature: Click in the Dwell Time or

Setpoint cell, and then enter the Dwell Time or Setpoint temperature. To enter 10

seconds, type 0 followed by a colon (:), then type 10 (that is, as the time appears in

the spreadsheet). Alternatively, 10 seconds can be entered as 0.10.

• Set Data Acquisition Step: Click in the Data Acquisition column, and then click

Real-Time at the step you want to collect real-time data. Click Melt Curve if data

from a melt curve is required.

NOTE: Ensure that every thermal protocol has at least one Data Acquisition step.

• Insert a cycle: Insert a cycle by clicking in the Insert column within the cycle row.

Cycles have a blue background. The iQ5 software inserts the new cycle below the

current cycle

• Insert a step: Insert a step by clicking in the Insert column within a step row.

Steps have a white background. The iQ5 software inserts the new step below the

current cycle. You can use the Options cell to customize whether the step or cycle is

inserted before or after the current cycle/step, as well as how many steps the iQ5

software will insert when you insert a cycle

• Delete a cycle: Delete a cycle by clicking in the Delete column within a cycle row.

Cycles are indicated with a blue background.

• Delete a step: Delete a step by clicking in the Delete column within a step row.

Steps are indicated with a white background

2. Save the protocol by clicking Save & Exit Protocol Editing. Enter the name of the

protocol in the Save As dialog box, and then click Save.

2.2 Plate Setup Quick Guide

Within the Workshop module:

1. Open the Plate Setup Editor Window using one of the following methods:

• Click Create New in the plate setup display pane to enter the Plate Setup Editor

• Click Plate and select the desired plate setup file from the file tree directory. Double-

click the file name to go directly to the Plate Setup Editor

• Click Plate and select the desired plate setup file from the file tree directory. Click

the file name to open the plate setup in the bottom right section of the Workshop