Software, Version 2.1 Owner's manual

Section 2 Quick Guides
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window. Click Edit to open the plate setup in the Plate Setup Editor
or
• Click Data File and select the desired data setup file from the file tree directory.
Click the file name to open the plate setup associated with the data file in the bottom
right section of the Workshop window. Click Edit to open the plate setup in the Plate
Setup Editor
2. Enter or edit any notes about the plate setup in the Notes box.
3. Enter or edit the sample volume, seal type, and vessel type.
4. Enter or edit a name for the experiment.
5. Click Select/Add Fluorophores and select or edit the fluorophores to be used on the
plate.
6. For most experiments, leave the Whole Plate Loading box checked. With Whole Plate
Loading, changes made to any fluorophore within a well are extended to the other
fluorophores within the well and the replicate group. If you are editing a plate, the Whole
Plate Loading checkbox may be unavailable because it is not appropriate based on the
current definition of the plate.
7. Click a sample type icon.
8. Select the type of replicate loading desired.
9. Click a fluorophore.
10. Click or drag across the plate to define wells with the selected fluorophore and sample
type.
11. Continue defining the remaining wells that will contain the first fluorophore by changing
to any other sample type icons required.
To calculate standard concentrations automatically, click Dilution Series and enter the
upper or lower concentrations, and units of the standards, set the dilution factor, and
click Apply Dilution Series.
12. Repeat steps 7–11 for any additional dye layers/fluorophores as required. Remember
that if the Whole Plate Loading box is checked, changes made in standard concentrations
will be applied to all dye layers for that well and extended to all replicates in the group.
NOTE: To delete a previously defined well, click the Delete All icon, and then click the
well. To delete the selected fluorophore from a previously defined well, click the Delete
Fluorophore icon, and then click the well.
13. Click Save & Exit Plate Editing.
2.3 Running a Real-Time Experiment Quick Guide
After you click Run in the Workshop module, the iQ5 software opens the Initiate Run tab within
the Run-Time Central module.
2.3.1 Beginning a Run
To begin a run:
1. Insert the experimental plate into the iCycler reaction module.