Software, Version 2.1 Owner's manual

Section 2 Introduction to the iQ5 Optical System Software
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Section 3. Introduction to the iQ5 Optical
System Software (Version 2.1)
The iQ5 software is divided into five sections, called modules. Icons representing each of the
modules are always shown on the left side of the screen. The active or selected module has an
orange background, whereas unselected modules have a gray background. Each module is
subdivided into windows that perform a specific function for that module.
The five modules in the iQ5 software are the:
Workshop Module: This module is used to select a Plate and Protocol and
Run an experiment. It is also where experimental files are selected and
opened for Data Analysis. The Workshop module consists of a Setup and
Plate Summary window. The Protocol, Plate, Run Set and Data File tabs can
be used to select, open, edit or create files. In Setup the selected Protocol
and Plate Setup can be run or the selected data file can be opened for
analysis in Data Analysis.
Run-Time Central Module: This module is used to initiate and monitor
experimental runs. It is accessed from the Workshop module, once the
Protocol and Plate Setup have been chosen, by clicking Run or Run End
Point.
Data Analysis Module: This module contains a suite of tools enabling you
to conduct thorough and varied analyses of your experimental data. Within
this module are screens for Quantitative, Melt Curve/Peak, End Point, Allelic
Discrimination, and Gene Expression analyses. The Edit Plate screen permits
post-run editing of the experimental plate setup, allowing you to correct
erroneous sample type assignments. The Data Analysis module is opened
automatically when you open a saved data file from the Workshop.
Calibration Module: In order to extract the best data from your real-time
PCR experiment, the MyiQ2, MyiQ or iQ5 systems must be calibrated. These
simple and easily-performed calibration routines are accessed in the
Calibration module. There are calibration routines for Pure Dyes, Mask
Alignment, Background, and Well Factors collection.
User Profile Module: This module can be used by the
Administrator to add
new users and to set users access/restrictions to various functions of the iQ5
software.
All users can use this module to set their personal preferences for the iQ5
software.