Software, Version 2.1 Owner's manual

Section 4 Workshop Module
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Fig. 4.6. List of Five Sets of Wells.
Click Select/Add Fluorophores to open the Fluor Selector box. From this box, choose up to five
fluorophores to be monitored. To remove a fluorophore from the list to be monitored, uncheck
the box next to its name.
To change the default color associated with a fluorophore, click the color box on the row with the
fluorophore name and choose a new color as shown below in Figure 4.7.
Fig. 4.7. The Fluor Selector Window for the MyiQ2 Real-Time PCR Detection System.
The iQ5 Optical System software automatically recognizes which instrument is detected and
displays only the fluorophores appropriate to that system for selection. Figure 4.7 illustrates the
fluor selector window when connected to a MyiQ2 instrument.
You may add new dyes to the list at any time by clicking Add New Fluor. When you add a new
dye, you must specify the filter to be used to monitor that fluorophore and perform a pure dye
calibration for that fluorophore.