Software, Version 2.1 Owner's manual

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Table of Contents
NOTICE TO PURCHASER ..................................................................................................i
Safety Information .........................................................................................................ii
Section 1. Getting Started ..............................................................................................1
1.1 The MyiQ™2 Real-Time PCR Detection System ................................................................1
1.2 Setting Up the System Hardware ....................................................................................1
1.2.1 System Checklist......................................................................................................1
1.2.2 Installing the Optical Reaction Module on the iCycler Chassis......................................2
1.2.3 Installing the Support Bracket ..................................................................................3
1.2.4 Installing the Optics Module .....................................................................................3
1.2.5 Connecting Power and Communication Cables to the System .....................................3
1.3 Installing the iQ5 Software .............................................................................................4
1.3.1 Installing the Camera Drivers ………………………………………………………………………………4
1.4 Recommended Computer Settings...................................................................................4
1.5 Calibrating the Instrument ..............................................................................................6
1.6 Compatibility with Earlier Versions of the iQ5 Optical System Software ..............................6
Section 2. Quick Guides..................................................................................................7
2.1 Protocol Quick Guide ......................................................................................................7
2.2 Plate Setup Quick Guide .................................................................................................8
2.3 Running a Real-Time Experiment Quick Guide .................................................................9
2.3.1 Beginning a Run ......................................................................................................9
2.3.2 Monitoring the Run ................................................................................................10
2.4 Data Analysis Quick Guides...........................................................................................10
2.4.1 PCR Quant Tab Quick Guide ...................................................................................10
2.4.2 End Point Quick Guide............................................................................................11
2.4.3 Allelic Discrimination Quick Guide – For Multiplex Data Only ........................................12
2.4.4 Gene Expression Quick Guide.....................................................................................13
2.4.5 Post-Run Plate Setup Editing Quick Guide ...................................................................16
Section 3. Introduction to the iQ5 Optical System Software (Version 2.1) .................18
Section 4. Workshop Module........................................................................................19
4.1 Setup Tab....................................................................................................................19
4.1.1 File Browser Section...............................................................................................20
4.1.2 Selected Protocol Area ...........................................................................................20
4.1.3 Selected Plate Setup Area ......................................................................................20