Software, Version 2.1 Owner's manual

Section 6 Data Analysis Module
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Fig. 6.18. The Ending Cycle Only Mode.
Setting the Threshold Manually
By default, the iQ5 software automatically calculates the threshold. You can override auto-
calculation in one of two ways:
• On the amplification chart, move the cursor over the displayed threshold line until the
cursor icon becomes a hand. Left-click on the displayed threshold line and drag the
threshold to the desired position
• From the Base Line Threshold Parameter dialog box, select User Defined in the Crossing
Threshold section at the bottom of the dialog box. Enter the desired value for the
threshold position, and then click OK
6.5.2 Amplification Plot Data Display Options
There are seven additional data analysis options in the amplification plot menu:
• Single Point
• All Candidates
• Adjust Graph
• Define Trace Style
• Display Data
• Restore Graph
• Show All Traces
Single Point
The Single Point mode of data presentation, in which the iQ5 software averages all data
collected at a particular step and then plots the average, is the default mode. For example, if the
iQ5 software collects four data points during the third repeat of an amplification cycle, the mean
of those 4 points is plotted at cycle 3. The alternative to viewing the data in Single Point mode is
to view the data in All Candidates mode.
All Candidates
In All Candidates mode, the iQ5 software plots every single data point that is collected. You
cannot use automated data analysis features when the data are presented in All Candidates
mode. This mode is unavailable when the analysis mode is PCR Base Line Subtracted Curve Fit.