User Manual

Chapter 1 QX100 Droplet Digital PCR System
QX100 Droplet Reader and QuantaSoft Software Instruction Manual4 |
The QX100 ddPCR system is compatible with hydrolysis probe (TaqMan) chemistry and can detect up to two
probes at a time (FAM and VIC, or FAM and HEX), using a dye deconvolution matrix in the software to ensure
target specificity. The system is not compatible with SYBR
®
Green or EvaGreen
®
chemistry; using these
chemistries or other unapproved Bio-Rad supermixes may harm the instrument and voids the warranty.
1.4 System Setup and General Operation Instructions

Connect the QX100 droplet generator and QX100 droplet reader to a power source using the power cords
provided. Leave 10" (5 cm) space clear behind and 5" (2.5 cm) clear to the right and left of each instrument
for proper ventilation

Connect the QX100 droplet reader to the laptop PC using the USB 2.0 cord provided. QuantaSoft software
is preinstalled on the laptop

Power on the QX100 droplet reader using the switch at the back. The status indicator turns solid green to
indicate power is on. The QX100 droplet generator is powered on by plugging it into a power source

Open and close the instruments by pressing the button on top of the green lid

The droplet reader requires droplet reader oil (catalog #186-3004). Please refer to Section 4.2.2 for
instructions on replacing droplet reader oil