Instruction manual

QX200 Droplet Reader and QuantaSoft Software Instruction Manual QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 3
Chapter 1 QX200 Droplet Digital PCR System
Table 1.2. Additional materials required.
Component Recommended Catalog # and Supplier
QX200 droplet generator
Reagents for probe detection
PCR supermix ddPCR supermix for probes (no dUTP) 186-3023, 186-3024, 186-3025
ddPCR supermix for probes 186-3010, 186-3026, 186-3027,
186-3028
One-Step RT-ddPCR supermix for probes 186-3021, 186-3022
Droplet generation oil Droplet generation oil for probes 186-3005
Control ddPCR buffer control kit for probes 186-3052
Reagents for EvaGreen detection
PCR supermix QX200
™
ddPCR
™
EvaGreen
®
supermix 186-4033, 186-4034, 186-4035,
186-4036
Droplet generation oil Droplet generation oil for EvaGreen 186-4005, 186-4006
Control QX200 buffer control kit for EvaGreen 186-4052
Consumables and other materials
Pipets 20 μl pipet for sample loading Rainin L-20
50 μl pipet for droplet transfer Rainin L-50, L8-50
8-channel, 200 μl pipet for oil Rainin L8-200
Pipet tips Filtered Rainin GP-L10F, GP-L200F
96-well PCR plates twin.tec semi-skirted 96-well plate Eppendorf 951020362
Reagent trough Any
Foil plate seals Pierceable foil plate seals 181-4040
Plate sealer PX1
™
PCR plate sealer 181-4000
8-cap strips Any
QX200 droplet reader
Droplet reader oil ddPCR droplet reader oil 186-3004
Droplet reader waste bottle Empty waste bottle N/A