Instruction manual

| 19QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Chapter 3 Using QuantaSoft Software
3.3.3 Viewing Concentration Data (Concentration)
Concentration data for each target appear in the wells in the plate map and are tabulated in the results table.
Click Concentration to visualize data in concentration plots. Use the radio buttons to select the channels
displayed. Error bars reflect total error or Poisson 95% confidence limits. These data can be exported for
analysis in other spreadsheet applications (for example, Microsoft Excel).
Toggle y-axis log scale
QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Display data from single wells, merged data, or both
Toggle x-axis values and well, name, or label display
Viewing concentration data. Data from absolute quantification are shown. Hover over data points to reveal well identity, concentration,
and Poisson confidence limits. Solid data points (shown) indicate merged data; open data points (not shown) indicate data from single
wells.
Select channel(s) to display
Y2 Axis options:
None — no Y2 axis
Ch2 — display Ch2 concentration
CNV — display copy number
(CNV analysis only)
Ratio(a/b) — ratio (a/b) of
unknown:reference
Fractional Abundance
(a/a+b) — ratio (a/[a+b]) of
unknown:reference; abundance
Inverse — inverse of ratio or
fractional abundance
The Copies/µL Well column displays the total amount of starting material in the ddPCR sample. The values
shown reflect the product of the concentration (in copies per µl) multiplied by the 20 µl ddPCR reaction input
used to make droplets. This value does not appear for merged well data.
This column is also exported in the *.csv file. The original concentration column and calculations remain
unchanged.