Agilent 1260 Infinity Bio-inert Quaternary Pump User Manual Agilent Technologies
Notices © Agilent Technologies, Inc. 2011-2012 Warranty No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws. The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions.
In This Guide... In This Guide... This manual covers the Agilent 1260 Infinity Bio-inert Quaternary Pump G5611A. 1 Introduction This chapter gives an introduction to the module, instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications. 3 Installing the Pump This chapter gives information about the preferred stack setup for your system and the installation of your module.
In This Guide... 8 Test Functions and Calibration This chapter describes the tests for the module. 9 Maintenance This chapter describes the maintenance of the module. 10 Parts for Maintenance This chapter provides information on parts for maintenance. 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. 12 Hardware Information This chapter describes the pump in more detail on hardware and electronics.
Contents Contents 1 Introduction 9 Introduction to the Pump 10 Overview of the Hydraulic Path 11 Bio-inert Materials 17 Early Maintenance Feedback 19 Instrument Layout 20 2 Site Requirements and Specifications 21 Site Requirements 22 Physical Specifications 25 Performance Specifications 26 3 Installing the Pump 27 Unpacking the Pump 28 Optimizing the Stack Configuration 30 Installing the Pump 33 Connecting Modules and Control Software Flow Connections of the Pump 38 Priming the System 41 4 Using the
Contents 6 Troubleshooting and Diagnostics 63 Overview of the Module’s Indicators and Test Functions Status Indicators 66 User Interfaces 68 Agilent Lab Advisor Software 69 7 Error Information 64 71 What Are Error Messages 73 General Error Messages 74 Module Error Messages 80 8 Test Functions and Calibration Introduction 96 System Pressure Test Leak Rate Test 102 9 Maintenance 95 97 107 Introduction to Maintenance 108 Warnings and Cautions 109 Overview of Maintenance 111 Cleaning the Module 112 Ch
Contents 10 Parts for Maintenance 137 Pump Head Assembly with Seal Wash Option Outlet Valve 140 Purge Valve Assembly 141 Active Inlet Valve 142 Accessory Kit, Bio-inert (G5611-68755) 144 Starter-Kit Bio-inert 145 Solvent Cabinet 146 Bottle Head Assembly 148 Hydraulic Path 149 System Tool Kit 151 11 Identifying Cables 138 153 Cable Overview 154 Analog Cables 156 Remote Cables 158 BCD Cables 161 CAN/LAN Cables 163 External Contact Cable 164 Agilent Module to PC 165 Agilent 1200 Module to Printer 12 Hard
Contents 8 Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 1 Introduction Introduction to the Pump 10 Overview of the Hydraulic Path 11 Hydraulic Path 12 How Does the Pump Work? 13 How Does Compressibility Compensation Work? How Does Variable Stroke Volume Work? 16 Bio-inert Materials 17 Early Maintenance Feedback Instrument Layout 15 19 20 This chapter gives an introduction to the module, instrument overview and internal connectors.
1 Introduction Introduction to the Pump Introduction to the Pump The bio-inert quaternary pump comprises an optional solvent cabinet, a vacuum degasser and a four-channel gradient pump. The four-channel gradient pump comprises a high-speed proportioning valve and a pump assembly. It provides gradient generation by low pressure mixing. A solvent cabinet provides enough space for four one-liter bottles.
Introduction Overview of the Hydraulic Path 1 Overview of the Hydraulic Path The bio-inert quaternary pump is based on a two-channel, dual-piston in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by one pump assembly which can generate pressure up to 600 bar. Degassing of the solvents is done in a built-in vacuum degasser.
1 Introduction Overview of the Hydraulic Path Hydraulic Path Figure 2 12 Hydraulic Path of the Bio-inert Quaternary Pump Bio-inert Quaternary Pump User Manual
Introduction Overview of the Hydraulic Path 1 KVXjjb X]VbWZg 9Z\VhhZg ;gdb hdakZci WdiiaZh 9VbeZg Id hVbea^c\ jc^i VcY Xdajbc >caZi kVakZ DjiaZi kVakZ Id lVhiZ Figure 3 Hydraulic Path of the Quaternary Pump How Does the Pump Work? In the pump, the liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve. The pump assembly comprises two substantially identical piston/chamber units.
1 Introduction Overview of the Hydraulic Path depending on the flow rate. The microprocessor controls all flow rates in a range of 1 µL/min – 10 mL/min. The inlet of the first pumping unit is connected to the active inlet valve. The outlet of the first piston/chamber unit is connected through the outlet valve and the damping unit to the inlet of the second piston/chamber unit. The outlet of the purge valve assembly is then connected to the following chromatographic system.
Introduction Overview of the Hydraulic Path 1 When turned on, the pump runs through an initialization procedure to determine the upper dead position of the first piston. The first piston moves slowly upwards into the mechanical stop of the pump chamber and from there it moves back for a defined distance. The controller stores this piston position in memory. After this initialization the pump starts operation with the set parameters.
1 Introduction Overview of the Hydraulic Path With a compressibility value set the processor calculates a compensation volume, that depends on the back pressure of the system and the selected compressibility. This compensation volume will be added to the normal stroke volume and compensates the previously described loss of volume during the delivery stroke of the first piston.
Introduction Bio-inert Materials 1 Bio-inert Materials For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses highest quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life scientists, as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range.
1 Introduction Bio-inert Materials Table 1 Bio-inert Materials Module Materials Bio-inert Flow Cells: Standard flow cell bio-inert, 10 mm, 13 µl, 120 bar (12 MPa) for MWD/DAD, includes Capillary Kit Flow Cells BIO (p/n G5615-68755) (p/n G5615-60022) (for Agilent 1260 Infinity Diode Array Detectors DAD G1315C/D) PEEK, zirconium oxide, sapphire, PTFE Max-Light Cartridge Cell Bio-inert (10 mm, V() 1.0 µl) (p/n G5615-60018) and Max-Light Cartridge Cell Bio-inert (60 mm, V() 4.
Introduction Early Maintenance Feedback 1 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.
1 Introduction Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed. This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet.
Bio-inert Quaternary Pump User Manual 2 Site Requirements and Specifications Site Requirements 22 Physical Specifications 25 Performance Specifications 26 This chapter provides information on environmental requirements, physical and performance specifications.
2 Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 2 on page 25. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.
2 Site Requirements and Specifications Site Requirements CAUTION Inaccessible power plug. In case of emergency it must be possible to disconnect the instrument from the power line at any time. ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Power Cords Different power cords are offered as options with the module. The female end of all power cords is identical.
2 Site Requirements and Specifications Site Requirements WA R N I N G Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment. ➔ Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. Bench Space The module dimensions and weight (see Table 2 on page 25) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.
2 Site Requirements and Specifications Physical Specifications Physical Specifications Table 2 Physical Specifications Type Specification Weight 14.5 kg (32 lbs) Dimensions (height × width × depth) 180 x 345 x 435 mm (7.0 x 13.
2 Site Requirements and Specifications Performance Specifications Performance Specifications Table 3 Specifications - Agilent 1260 Infinity Bio-inert Quaternary Pump (G5611A) Type Specifications Hydraulic system Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve, integrated 4-channel degassing unit Setable flow range 0.001 – 10 mL/min, in 0.001 mL/min increments Recommended flow range 0.2 – 10 mL/min Flow precision < 0.
Bio-inert Quaternary Pump User Manual 3 Installing the Pump Unpacking the Pump 28 Delivery Checklist 28 Accessory Kit 29 Optimizing the Stack Configuration One Stack Configuration 30 Installing the Pump 30 33 Connecting Modules and Control Software 36 Connecting Modules 36 Connecting Control Software and/or G4208 A Instant Pilot Flow Connections of the Pump 37 38 Priming the System 41 Initial Priming 41 Regular Priming 43 Changing Solvents 44 This chapter gives information about the preferred stack
3 Installing the Pump Unpacking the Pump Unpacking the Pump If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. CAUTION "Defective on arrival" problems If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.
Installing the Pump Unpacking the Pump p/n Description 699975-902 Column Poroshell 120 EC-C18, 4.6 x 50 mm, 2.7 µm (optional) 883975-902 Column SB-C18, 4.6 x 150 mm, 5 µm (optional) G1369-60002 LAN Communication Card 3 Power cord Accessory Kit Accessory Kit (Bio-inert) (p/n G5611-68755) p/n Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m G5611-60502 Capillary 900 x 0.
3 Installing the Pump Optimizing the Stack Configuration Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.
Installing the Pump Optimizing the Stack Configuration 3 HdakZci XVW^cZi Ejbe AdXVa jhZg ^ciZg[VXZ 6jidhVbeaZg I]ZgbdhiViiZY Xdajbc XdbeVgibZci 9ZiZXidg Figure 5 Recommended Stack Configuration (Front View) Bio-inert Quaternary Pump User Manual 31
3 Installing the Pump Optimizing the Stack Configuration 68 edlZg 86C Wjh XVWaZ id adXVa jhZg ^ciZg[VXZ GZbdiZ XVWaZ 86C Wjh XVWaZ A6C id Xdcigda hd[ilVgZ adXVi^dc YZeZcYh dc YZiZXidg 6cVad\ YZiZXidg h^\cVa & dg ' djiejih eZg YZiZXidg Figure 6 32 Recommended Stack Configuration (Rear View) Bio-inert Quaternary Pump User Manual
Installing the Pump Installing the Pump 3 Installing the Pump Parts required # p/n 1 Pump 1 1 Description Data System G4208A 1 Instant Pilot Power cord For other cables see text below and “Cable Overview” on page 154. Preparations WA R N I N G • • • Locate bench space. Provide power connections. Unpack the module. Module is partially energized when switched off, as long as the power cord is plugged in. Repair work at the module can lead to personal injuries, e.g.
3 Installing the Pump Installing the Pump 1 Place the module on the bench in a horizontal position. 2 Ensure the power switch on the front of the module is OFF (switch stands out). HiVijh aVbe CVbZ eaViZ EdlZg hl^iX] HZg^Va cjbWZg Figure 7 Front of Pump 3 Connect the power cable to the power connector at the rear of the module.
3 Installing the Pump Installing the Pump 4 Connect the required interface cables to the quaternary pump, see “Connecting Modules and Control Software” on page 36. 8dc[^\jgVi^dc hl^iX] Hadi [dg ^ciZg[VXZ WdVgY 6cVad\ egZhhjgZ 6E< gZbdiZ GH"'('8 86C EdlZg 5 Connect all capillaries, solvent tubes and waste tubing (see “Flow Connections of the Pump” on page 38). 6 Press the power switch to turn on the module.
3 Installing the Pump Connecting Modules and Control Software Connecting Modules and Control Software WA R N I N G Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
3 Installing the Pump Connecting Modules and Control Software Connecting Control Software and/or G4208 A Instant Pilot NOTE With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. The preferred communication is LAN. NOTE Usually the detector is producing the most data in the stack, followed by the pump, and it is therefore highly recommended to use either of these modules for the LAN connection.
3 Installing the Pump Flow Connections of the Pump Flow Connections of the Pump Parts required Preparations WA R N I N G # Description 1 Other modules 1 Parts from accessory kit 2 wrenches 1/4 - 5/16 inch for capillary connections Pump is installed in the LC system Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks.
3 Installing the Pump Flow Connections of the Pump 1 Remove the front cover by pressing the snap fasteners on both sides. 2 Place the solvent cabinet on top of the quaternary pump. 3 Put the bottle-head assemblies into solvent reservoirs containing your mobile phase and place the bottle in the solvent cabinet. 4 Connect the inlet tubes from the bottle-head assemblies to the inlet connectors A to D (typically the left connection of the channel) of the vacuum degasser.
3 Installing the Pump Flow Connections of the Pump 9 Prime your system before first use (see “Initial Priming” on page 41 ).
3 Installing the Pump Priming the System Priming the System Initial Priming When Before a new degasser or new solvent tubing can be used, it is necessary to prime the system. Isopropanol (IPA) is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties. Parts required # Description 1 Isopropanol Preparations Connect all modules hydraulically as described in the respective module manuals.
3 Installing the Pump Priming the System NOTE When priming the vacuum degasser with a syringe, the solvent is drawn through the degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully degassed. Pump for approximately 10 minutes at your desired flow rate before starting an analysis. This will allow the vacuum degasser to properly degas the solvent in the degasser tubes. 1 Open the purge valve of the pump 2 Set the flow rate to 5 mL/min.
3 Installing the Pump Priming the System Regular Priming When When the pumping system has been turned off for a certain time (for example, overnight) air will rediffuse into the solvent channel between the vacuum degasser and the pump. Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time.
3 Installing the Pump Priming the System Changing Solvents When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents are immiscible or one solvent contains a buffer) it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
Installing the Pump Priming the System Table 4 3 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation When switching between reverse phase and normal phase (both times) Isopropanol Isopropanol Best solvent to flush air out of the system Miscible with almost all solvents After an installation Ethanol or methanol Alternative to isopropanol (second choice) if no isopropanol is available To clean the system when using buffers After changing aqueous solven
3 46 Installing the Pump Priming the System Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 4 Using the Pump Hints for Successful Use of the Quaternary Pump Prevent Blocking of Solvent Filters 48 49 Algae Growth in HPLC Systems 50 How to Prevent and/or Reduce the Algae Problem Solvent Information 50 51 This chapter explains the operational parameters of the module.
4 Using the Pump Hints for Successful Use of the Quaternary Pump Hints for Successful Use of the Quaternary Pump • Always place the solvent cabinet with the solvent bottles on top of the quaternary pump (or at a higher level). • When using salt solutions and organic solvents in the quaternary pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports.
Using the Pump Prevent Blocking of Solvent Filters 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module. This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7). The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module.
4 Using the Pump Algae Growth in HPLC Systems Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems. Algae grow in aqueous media, preferably in a pH range of 4-8. Their growth is accelerated by buffers, for example phosphate or acetate. Since algae grow through photosynthesis, light will also stimulate their growth. Even in distilled water small-sized algae grow after some time.
Using the Pump Solvent Information 4 Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see “Algae Growth in HPLC Systems” on page 50 • Small particles can permanently block capillaries and valves. Therefore always filter solvents through 0.4 µm filters. • Avoid or minimize the use of solvents, which may corrode parts in the flow path.
4 Using the Pump Solvent Information PEEK PEEK (Polyether-Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability and is therefore the material of choice for biochemical instrumentation. It is stable in the specified pH range and inert to many common solvents.
Using the Pump Solvent Information 4 Gold Gold is inert against all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia (a mixture of concentrated hydrochloric and nitric acid). Zirconium Oxide Zirconium Oxide (ZrO2) is inert against almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
4 54 Using the Pump Solvent Information Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 5 Optimizing Performance When to Use a Degasser 56 Operational Hints for the Multi Channel Gradient Valve (MCGV) When to use the Seal Wash Option Choosing the Right Pump Seals 57 58 59 Optimize the Compressibility Compensation Setting 60 This chapter gives hints on how to optimize the performance or use additional devices.
5 Optimizing Performance When to Use a Degasser When to Use a Degasser The bio-inert quaternary pump has a built-in degasser, which should always be included to the flow path. Operational Hints for the Internal Degasser If you are using the degasser for the first time, if the degasser was switched off for some time (for example, overnight), or if the degasser lines are empty, you should prime the degasser before running an analysis.
5 Optimizing Performance Operational Hints for the Multi Channel Gradient Valve (MCGV) Operational Hints for the Multi Channel Gradient Valve (MCGV) In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations. However in the mixing point of the gradient valve, at the boundary between the two solvents, micro precipitation is possible. Gravity forces the salt particles to fall down.
5 Optimizing Performance When to use the Seal Wash Option When to use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent. The seal wash option is strongly recommended when buffer concentrations of 0.1 M or higher will be used for long time periods in the pump.
Optimizing Performance Choosing the Right Pump Seals 5 Choosing the Right Pump Seals The standard seal for the pump can be used for most applications. However applications that use normal phase solvents (for example, hexane) are not suited for the standard seal and require a different seal when used for a longer time in the pump. For applications that use normal phase solvents (for example, hexane) we recommend using bio-inert PE piston seals and bio-inert PE wash seals.
5 Optimizing Performance Optimize the Compressibility Compensation Setting Optimize the Compressibility Compensation Setting The compressibility compensation default setting is 100 × 10-6 /bar for the pump. This setting represents an average value. Under normal conditions the default setting typically reduces the pressure pulsation to values below 1 % of system pressure that are sufficient for most applications and for all gradient analyses.
Optimizing Performance Optimize the Compressibility Compensation Setting Table 5 5 Solvent Compressibility Solvent (pure) Compressibility (10-6/bar) Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 Isopropanol 100 Methanol 120 1-Propanol 100 Toluene 87 Water 46 Bio-inert Quaternary Pump User Manual 61
5 62 Optimizing Performance Optimize the Compressibility Compensation Setting Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions 64 Status Indicators 66 Power Supply Indicator 66 Module Status Indicator 67 User Interfaces 68 Agilent Lab Advisor Software 69 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.
6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Overview of the Module’s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the module. The status indicators provide a quick visual check of the operation of the module.
Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions 6 System Pressure Test The System Pressure Test is a quick test designed to determine the pressure tightness of the system (i.e. the high pressure flow path between pump and column). After exchanging flow path components (e.g. pump seals or injection seal), use this test to verify the system is pressure tight, see “System Pressure Test” on page 97.
6 Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status. HiVijh ^cY^XVidg EdlZg hjeean ^cY^XVidg Figure 9 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON.
6 Troubleshooting and Diagnostics Status Indicators Module Status Indicator The module status indicator indicates one of six possible module conditions: • When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis. • A green status indicator, indicates the module is performing an analysis (run mode). • A yellow indicator indicates a not-ready condition.
6 Troubleshooting and Diagnostics User Interfaces User Interfaces Depending on the user interface, the available tests vary. Some descriptions are only available in the service manual. Table 6 68 Test functions available vs. user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Yes (B.02.11) Yes (B.01.04) Leak Rate Test No Yes (B.01.04.
Troubleshooting and Diagnostics Agilent Lab Advisor Software 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet. Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules.
6 70 Troubleshooting and Diagnostics Agilent Lab Advisor Software Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 7 Error Information What Are Error Messages 73 General Error Messages 74 Timeout 74 Shut-Down 74 Remote Timeout 75 Synchronization Lost 76 Leak 76 Leak Sensor Open 77 Leak Sensor Short 77 Compensation Sensor Open Compensation Sensor Short Fan Failed 79 Open Cover 79 78 78 Module Error Messages 80 Restart Without Cover 80 Solvent Zero Counter 80 Pressure Above Upper Limit 81 Pressure Below Lower Limit 82 Pressure Signal Missing 82 Missing Pressure Reading 83 Pump Co
7 Error Information Agilent Lab Advisor Software Temperature Limit Exceeded 88 Servo Restart Failed 89 Pump Head Missing 89 Index Limit 90 Index Adjustment 90 Index Missing 91 Stroke Length 91 Initialization Failed 92 Wait Timeout 93 Degasser: cannot read signal 94 Degasser: limit not reached 94 This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
7 Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook.
7 Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout The timeout threshold was exceeded. Probable cause Suggested actions 1 The analysis was completed successfully, Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required. and the timeout function switched off the module as requested.
Error Information General Error Messages Probable cause Suggested actions 3 Shut-down in an external instrument with a Check external instruments for a shut-down condition. remote connection to the system. 4 The degasser failed to generate sufficient vacuum for solvent degassing. 7 Check the vacuum degasser for an error condition. Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built-in. Remote Timeout A not-ready condition is still present on the remote input.
7 Error Information General Error Messages Synchronization Lost During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated. Probable cause Suggested actions 1 CAN cable disconnected. • Ensure all the CAN cables are connected correctly.
Error Information General Error Messages 7 Leak Sensor Open The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits. If the current falls outside the lower limit, the error message is generated. Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service representative.
7 Error Information General Error Messages Compensation Sensor Open The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor increases above the upper limit, the error message is generated.
7 Error Information General Error Messages Fan Failed The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated. This limit is given by 2 revolutions/second for longer than 5 seconds. Probable cause Suggested actions 1 Fan cable disconnected. Please contact your Agilent service representative. 2 Defective fan.
7 Error Information Module Error Messages Module Error Messages These errors are pump specific. Restart Without Cover The module was restarted with the top cover and foam open. The sensor on the main board detects when the top foam is in place. If the module is restarted with the foam removed, the module switches off within 30 s, and the error message is generated. Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service representative.
Error Information Module Error Messages 7 Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit. Probable cause Suggested actions 1 Upper pressure limit set too low. Ensure the upper pressure limit is set to a value suitable for the analysis. 2 Blockage in the flowpath (after the damper). Check for blockage in the flowpath.
7 Error Information Module Error Messages Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit. Probable cause Suggested actions 1 Lower pressure limit set too high. Ensure the lower pressure limit is set to a value suitable for the analysis. 2 Air bubbles in the mobile phase. • Ensure solvents are degassed. Purge the module. • Ensure solvent inlet filters are not blocked. • Inspect the pump head, capillaries and fittings for signs of a leak.
Error Information Module Error Messages 7 Missing Pressure Reading The pressure readings read by the pump ADC (analog-digital converter) are missing. The ADC reads the pressure signal of from the damper every 1ms. If the readings are missing for longer than 10 seconds, the error message is generated. Probable cause Suggested actions 1 Damper disconnected. Please contact your Agilent service representative. 2 Defective damper. Please contact your Agilent service representative.
7 Error Information Module Error Messages Valve Fuse Valve Fuse 0: Channels A and B Valve Fuse 1: Channels C and D The gradient valve in the quaternary pump has drawn excessive current causing the electronic fuse to open. Probable cause Suggested actions 1 Defective gradient valve. Restart the quaternary pump. If the error message appears again, exchange the gradient valve. 2 Defective connection cable (front panel to Please contact your Agilent service representative. main board).
7 Error Information Module Error Messages Valve Failed (MCGV/SSV) Valve 0 Failed: valve A Valve 1 Failed: valve B Valve 2 Failed: valve C Valve 3 Failed: valve D One of the valves of the multi-channel gradient valve has failed to switch correctly. The processor monitors the valve voltage before and after each switching cycle. If the voltages are outside expected limits, the error message is generated. Probable cause Suggested actions 1 Gradient valve disconnected.
7 Error Information Module Error Messages Motor-Drive Power The current drawn by the pump motor exceeded the maximum limit. Blockages in the flow path are usually detected by the pressure sensor in the damper, which result in the pump switching off when the upper pressure limit is exceeded. If a blockage occurs before the damper, the pressure increase cannot be detected by the pressure sensor and the module will continue to pump. As pressure increases, the pump drive draws more current.
Error Information Module Error Messages 7 Encoder Missing The optical encoder on the pump motor in the module is missing or defective. The processor checks the presence of the pump encoder connector every 2 seconds. If the connector is not detected by the processor, the error message is generated. Probable cause Suggested actions 1 Defective or disconnected pump encoder Please contact your Agilent service representative. connector. 2 Defective pump drive assembly.
7 Error Information Module Error Messages Temperature Out of Range The temperature sensor readings in the motor-drive circuit are out of range. The values supplied to the ADC by the hybrid sensors must be between 0.5 V and 4.3 V. If the values are outside this range, the error message is generated. Probable cause Suggested actions 1 Defective main board. Please contact your Agilent service representative. Temperature Limit Exceeded The temperature of one of the motor-drive circuits is too high.
7 Error Information Module Error Messages Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting. When the module is switched on, the first step is to switch on the C phase of the variable reluctance motor. The rotor should move to one of the C positions. The C position is required for the servo to be able to take control of the phase sequencing with the commutator.
7 Error Information Module Error Messages Index Limit The time required by the piston to reach the encoder index position was too short (pump). During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached. If the index position is reached too fast, the error message is generated. Probable cause Suggested actions 1 Irregular or sticking drive movement.
7 Error Information Module Error Messages Index Missing The encoder index position in the module was not found during initialization. During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached. If the index position is not recognized within a defined time, the error message is generated. Probable cause Suggested actions 1 Disconnected or defective encoder cable.
7 Error Information Module Error Messages Initialization Failed The module failed to initialize successfully within the maximum time window. A maximum time is assigned for the complete pump-initialization cycle. If the time is exceeded before initialization is complete, the error message is generated. 92 Probable cause Suggested actions 1 Blocked active inlet valve. Exchange the active inlet valve. 2 Defective pump drive assembly. Please contact your Agilent service representative.
Error Information Module Error Messages 7 Wait Timeout When running certain tests in the diagnostics mode or other special applications, the pump must wait for the pistons to reach a specific position, or must wait for a certain pressure or flow to be reached. Each action or state must be completed within the timeout period, otherwise the error message is generated. Possible Reasons for a Wait Timeout: · Pressure not reached. · Pump channel A did not reach the delivery phase.
7 Error Information Module Error Messages Degasser: cannot read signal The pump board gets no or wrong pressure signals from the built-in degasser. Probable cause Suggested actions 1 Degasser board defect, missing or not Please contact your Agilent service representative. connected to the pump main board. 2 Degasser sensor defect or not connected to degasser board Please contact your Agilent service representative.
Bio-inert Quaternary Pump User Manual 8 Test Functions and Calibration Introduction 96 System Pressure Test 97 Running the Test 99 Evaluating the Results 99 System Pressure Test failed 101 Leak Rate Test 102 Running the Test 104 Evaluating the Results 104 Potential Causes of Leak Rate Test Failure 105 This chapter describes the tests for the module.
8 Test Functions and Calibration Introduction Introduction All tests are described based on the Agilent Lab Advisor Software B.01.04. Other user interfaces may not provide all tests listed here. For details on the use of the interface refer to the interface documentation.
Test Functions and Calibration System Pressure Test 8 System Pressure Test Introduction The System Pressure Test is used for checking the tightness of the LC system and identifying leaks between the pump and a position in the flow path following the pump blocked by a blank nut. Test Principle A solvent can be chosen from available solvent channels and a maximum pressure can be defined at which the test will be run. In contrast to older revisions of this test, any solvent can be used.
8 Test Functions and Calibration System Pressure Test egZhhjgZ ' & i^bZ [adl aZV` gViZ i^bZ In the first phase of the test, the pump delivers flow at a rate of 200 µL until a pressure of 50 bar below the defined maximum pressure is reached. In the second phase, the pump delivers a small flow which is increased stepwise. If there is a leak in the system, the pressure will drop initially, as the low flow cannot compensate the leak flow.
8 Test Functions and Calibration System Pressure Test Running the Test Parts required CAUTION p/n Description 01080-83202 Blank nut Damage to pressure sensitive parts Even columns that are suitable for high pressures are sensitive to pressure drops that occur during this test. ➔ Do not include any pressure sensitive parts to the flow path and choose a maximum pressure that is compatible to your system.
8 Test Functions and Calibration System Pressure Test NOTE Often it is only a damaged blank nut itself (poorly shaped from overtightening) that causes a failure of the test. Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened! If the test fails again, insert the blank nut at the outlet of the previous module in the stack (e.g. autosampler outlet if TCC has been tested before), and repeat the test.
Test Functions and Calibration System Pressure Test 8 System Pressure Test failed The test will fail, if the sum of all leaks in the system (pump, autosampler or column compartment and connections) exceeds the test limit. After isolating and fixing the cause of the leak, repeat the System Pressure Test to confirm the system is pressure tight. Probable cause Suggested actions 1 Purge valve open. Close the purge valve. 2 Loose or leaky fittings. Tighten the fitting or exchange the capillary.
8 Test Functions and Calibration Leak Rate Test Leak Rate Test Introduction The Leak Rate Test is used for verifying the internal tightness of the pump and helps identifying parts which may have caused a leak. System requirements Minimum software revisions: • Lab Advisor B.01.04. SP1 (G1310B 1260 Isocratic Pump, G1311B 1260 Quaternary Pump, G4280B 1220 Isocratic Pump, G4281B 1220 Gradient Pump) • Lab Advisor B.01.04.
Test Functions and Calibration Leak Rate Test 8 Initially, the pressure is increased to about 100 bar below the target pressure, which has been set for the test. Then piston 1 is brought to its rear position. An increasing flow is delivered by piston 1. In case of a leak, the pressure will drop initially as long as the flow rate delivered by the piston is lower than the leak rate. As soon as the flow rate of the piston exceeds the leak rate, the measured pressure will increase again.
8 Test Functions and Calibration Leak Rate Test Running the Test Parts required p/n Description 01080-83202 Blank nut Running the test from the Agilent Lab Advisor 1 Select the Leak Rate Test from the Test Selection menu. 2 Start the test and follow the instructions. NOTE Make sure to release the pressure by slowly opening the purge valve when the test has finished.
8 Test Functions and Calibration Leak Rate Test Potential Causes of Leak Rate Test Failure Secondary Leak If a leak is found for movement of piston 2 (secondary leak), the following reasons are possible: Probable cause Suggested actions 1 System not flushed properly Flush system for several minutes 2 Degassing efficiency is low Check degasser performance 3 Purge valve not closed or defect Check purge valve 4 Blank nut not installed tightly Tighten or replace blank nut 5 Outlet valve leaking (rea
8 106 Test Functions and Calibration Leak Rate Test Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 9 Maintenance Introduction to Maintenance Warnings and Cautions 109 Overview of Maintenance Cleaning the Module 108 111 112 Checking and Replacing the Solvent Filter 113 Exchanging the Active Inlet Valve (AIV) or its Cartridge Exchanging the Outlet Valve 117 Exchanging the Purge Valve Frit or the Purge Valve Removing the Pump Head Assembly Seal Wear-in Procedure 114 119 122 125 Maintenance of the Pump Head 126 Reinstalling the Pump Head Assembly 129
9 Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for easy repair. The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack.
9 Maintenance Warnings and Cautions Warnings and Cautions WA R N I N G Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
9 Maintenance Warnings and Cautions CAUTION Safety standards for external equipment ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination.
9 Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the pump that can be carried out without opening the main cover.
9 Maintenance Cleaning the Module Cleaning the Module The module case should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent. Do not use an excessively damp cloth as liquid could drip into the module. WA R N I N G Liquid dripping into the electronic compartment of your module. Liquid in the module electronics can cause shock hazard and damage the module. ➔ Do not use an excessively damp cloth during cleaning.
9 Maintenance Checking and Replacing the Solvent Filter Checking and Replacing the Solvent Filter A functional solvent filter is essential for a good pump performance and for protecting the LC system. When If solvent filter is blocked. Parts required p/n Description 5041-2168 Solvent inlet filter, 20 µm pore size See “Bottle Head Assembly” on page 148 for related parts. CAUTION Small particles can permanently block the capillaries and valves of the module. Damage of the module.
9 Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge Exchanging the Active Inlet Valve (AIV) or its Cartridge When If internally leaking (backflow) Tools required Description Wrench, 14 mm Pair of tweezers Parts required Preparations p/n Description G5611-60025 Active Inlet Valve (AIV, Bio-inert), without cartridge G5611-60020 Cartridge for AIV (Bio-inert) G1311-67304 Connecting tube, MCGV to AIV • • Switch off pump at the main power switch and unplug the power cable.
9 Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head. 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly. 7 Before inserting the new valve cartridge clean the area in the actuator assembly. Flush the cartridge area thoroughly with alcohol.
9 Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge 13 Reconnect the solvent inlet tube to the adapter. Reconnect the active inlet valve cable to the connector in the Z-panel. 14 Reinstall the front cover. 15 Purge the system with 30 mL of solvent in order to achieve a low pressure ripple, see “Regular Priming” on page 43.
Maintenance Exchanging the Outlet Valve 9 Exchanging the Outlet Valve When If internally leaking Tools required p/n Description 8710-0510 Wrench open 1/4 — 5/16 inch 8710-1924 Wrench open 14 mm p/n Description G5611-60067 Outlet Valve (Bio-inert) Parts required Preparations NOTE • • Switch off pump at the main power switch Remove the front cover Before exchanging the outlet valve you can try to clean it in an ultrasonicator for 5 – 10 min.
9 Maintenance Exchanging the Outlet Valve 1 Using the 1/4 inch wrench disconnect the valve capillary 2 Do not disassemble the outlet valve, as this can damage from the outlet valve. Using the 14 mm wrench loosen the valve and remove it from the pump body. the valve. KVakZ XVe^aaVgn DjiaZi kVakZ NOTE The bio-inert outlet valve can be identified by a ring, which is normally covered by the plastic jacket. 118 3 Reinstall the outlet valve and tighten the valve. Reconnect the valve capillary.
Maintenance Exchanging the Purge Valve Frit or the Purge Valve 9 Exchanging the Purge Valve Frit or the Purge Valve When • • Tools required Frit – when piston seals are exchanged or when contaminated or blocked (pressure drop of > 10 bar across the frit at a flow rate of 5 mL/min of water with purge valve opened) Purge valve – if internally leaking p/n Description 8710-0510 Wrench open 1/4 — 5/16 inch 8710-1924 Wrench open 14 mm Pair of tweezers OR Parts required Preparations Toothpick # p/n
9 Maintenance Exchanging the Purge Valve Frit or the Purge Valve 1 Using a 1/4 inch wrench disconnect the pump outlet capillary from the purge valve. Disconnect the waste tube. Beware of leaking solvents due to hydrostatic pressure. 3 Remove the seal cap from the purge valve. 120 2 Using the 14 mm wrench unscrew the purge valve and remove it. 4 Using a pair of tweezers or a toothpick remove the frit.
9 Maintenance Exchanging the Purge Valve Frit or the Purge Valve 5 Place a new frit into the purge valve with the orientation of the frit as shown below (slit in frit points to the front). Reinstall the seal cap including the gold seal. 6 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube. NOTE Before reinstallation always check the gold seal in the seal cap. A deformed seal cap should be exchanged.
9 Maintenance Removing the Pump Head Assembly Removing the Pump Head Assembly When • • • Exchanging the seals Exchanging the pistons Exchanging seals of the seal wash option Tools required p/n Description Wrench, 1/4 inch 8710-2392 Preparations CAUTION • • Hexagonal key, 4.0 mm, 15 cm long, T-handle Switch off pump at the main power switch and unplug the power cable. Use a solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages.
Maintenance Removing the Pump Head Assembly 1 Remove the front cover by pressing the clip fasteners on 9 2 Disconnect the active inlet valve cable. both sides of the cover. 3 Using a 1/4 inch wrench remove the outlet capillary. 4 Remove the waste tubing and disconnect the solvent tubing from the inlet valve. 5 Remove the capillary at the bottom of the pump head.
9 Maintenance Removing the Pump Head Assembly 6 Using a 4 mm hexagonal key, stepwise loosen the two pump head screws and remove the pump head from the pump drive.
9 Maintenance Seal Wear-in Procedure Seal Wear-in Procedure Parts required CAUTION p/n Description 0100-1847 Adapter AIV to solvent inlet tubes 5022-2159 Restriction capillary Seal damage ➔ This procedure is required for Standard seals (pack of 2) (p/n 5063-6589), but it will damage the PE seals. NOTE The stainless steel restriction capillary is acceptable for a temporary test within a bio-inert system. In case of concerns, a PEEK capillary (e.g. PEEK capillary, 150 cm length, 0.13 mm i.d.
9 Maintenance Maintenance of the Pump Head Maintenance of the Pump Head When When maintaining seal wash option Tools required p/n Description 8710-2392 Hexagonal key, 4.0 mm, 15 cm long, T-handle p/n Description 01018-23702 Insert tool 0905-1731 Wash Seal PTFE for Bio-inert Pump 600 bar Parts required Preparations CAUTION 01018-07102 Gasket (Seal wash) G5611-21503 Piston Seal PTFE (Bio-inert) 5067-4695 Sapphire piston • • • • • Switch off pump at the main power switch.
Maintenance Maintenance of the Pump Head 1 Remove the seal holder and the seal wash support rings from the piston housing. Remove the seal holder from the support ring assembly. 3 Using the plastic side of the insert tool press the wash seal (spring pointing upwards) into the recess of the support ring. Bio-inert Quaternary Pump User Manual 9 2 Using the steel side of the insert tool remove the seal wash gasket and the secondary seal from the support ring.
9 Maintenance Maintenance of the Pump Head 5 Place a seal wash gasket in the recess of the support ring. 6 Place the support rings on the piston housing (pistons not Put the seal holder on top of the gasket. installed) and snap the pump head and piston housing together. Note the correct position of the pin on the support ring. 7 Insert the pistons and carefully press them into the seals. 8 Tighten the lock screw.
9 Maintenance Reinstalling the Pump Head Assembly Reinstalling the Pump Head Assembly When When reassembling the pump Tools required p/n Description 8710-2392 Hexagonal key, 4.0 mm, 15 cm long, T-handle p/n Description G5611-60165 Pump Head with Seal Wash Option (Bio-inert) 79846-65501 Pump head grease Parts required 1 If needed, apply a small amount of grease on the back of the screws. Normally, the grease added during manufacturing is sufficient for a long time.
9 Maintenance Reinstalling the Pump Head Assembly 3 Reconnect all capillaries, tubes and (if installed) the 4 Reinstall the front cover. active inlet valve cable to its connector.
Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) 9 Exchanging the Multi-Channel Gradient Valve (MCGV) Tools required Parts required Preparations NOTE p/n Description 8710-0899 Screwdriver, Pozidriv #1 p/n Description G5611-67701 Multi-Channel Gradient Valve (MCGV) (Bio-inert) • • • Switch off pump at the main power switch Remove the front cover Use a solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages.
9 Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) 1 Disconnect the connecting tube, waste tube and the solvent tubes from the MCGV. 2 Press the lower sides of the cover to unclip it. Remove the cover. LVhiZ [jccZa B8
Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) 4 Place the new MCGV into position. Make sure that 9 5 Install the MCGV cover. channel A of the MCGV is put at the bottom-right position. Tighten the two screws and connect the cable to its connector. 6 Reconnect the waste funnel with the waste tube holder in 7 Reconnect the tube from the inlet valve to the middle the top cover. Insert waste tube in the holder in the leak pan and clip tube to the MCGV cover.
9 Maintenance Exchanging the Optional Interface Board Exchanging the Optional Interface Board When Board defective Parts required # p/n Description 1 G1351-68701 Interface board (BCD) with external contacts and BCD outputs CAUTION Electronic boards are sensitive to electrostatic discharge (ESD) and should be handled with care so as not to damage them. Touching electronic boards and components can cause electrostatic discharge. ESD can damage electronic boards and components.
9 Maintenance Exchanging the Optional Interface Board 1 Switch off the pump at the main power switch, unplug the pump from line power. 2 Disconnect cables from the interface board connectors. 3 Loosen the screws. Slide out the interface board from the pump. 789 ^ciZg[VXZ WdVgY Figure 10 Exchanging the Interface Board 4 Install the new interface board. Secure screws. 5 Reconnect the cables to the board connector. 6 Reconnect the pump to line power.
9 Maintenance Replacing the Module’s Firmware Replacing the Module’s Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary • to keep all systems on the same (validated) revision or • if a new module with newer firmware is added to a system or • if third part control software requires a special version.
Bio-inert Quaternary Pump User Manual 10 Parts for Maintenance Pump Head Assembly with Seal Wash Option Outlet Valve 138 140 Purge Valve Assembly Active Inlet Valve 141 142 Accessory Kit, Bio-inert (G5611-68755) Starter-Kit Bio-inert Solvent Cabinet 144 145 146 Bottle Head Assembly Hydraulic Path 149 System Tool Kit 151 148 This chapter provides information on parts for maintenance.
10 Parts for Maintenance Pump Head Assembly with Seal Wash Option Pump Head Assembly with Seal Wash Option For bio-inert modules use bio-inert parts only! Item p/n 138 Description G5611-60165 Pump Head with Seal Wash Option (Bio-inert) 1 5067-4695 Sapphire piston 2 G1312-60062 Piston housing (incl. spring) 3 G5611-63010 Support Ring with Seal Wash Option (Bio-inert) 4 0905-1731 Wash Seal PTFE for Bio-inert Pump 600 bar 5065-9978 Silicone tubing, 1 mm i.d., 3 mm o.d.
Parts for Maintenance Pump Head Assembly with Seal Wash Option 10 &* & && ' &' ( * ) + - &) , .
10 Parts for Maintenance Outlet Valve Outlet Valve For bio-inert modules use bio-inert parts only! p/n Description G5611-60067 Outlet Valve (Bio-inert) Figure 12 140 Outlet valve, bio-inert Bio-inert Quaternary Pump User Manual
Parts for Maintenance Purge Valve Assembly 10 Purge Valve Assembly For bio-inert modules use bio-inert parts only! Item p/n Description 1 G5611-60061 Purge Valve (Bio-inert) 2 01018-22707 PTFE frits (pack of 5) 3 5067-4728 Seal cap Figure 13 Purge valve assembly, bio-inert Bio-inert Quaternary Pump User Manual 141
10 Parts for Maintenance Active Inlet Valve Active Inlet Valve For bio-inert modules use bio-inert parts only! 142 p/n Description G5611-60025 Active Inlet Valve (AIV, Bio-inert), without cartridge G5611-60020 Cartridge for AIV (Bio-inert) 0100-1847 Adapter AIV to solvent inlet tubes Bio-inert Quaternary Pump User Manual
Parts for Maintenance Active Inlet Valve Figure 14 10 Active inlet valve Bio-inert Quaternary Pump User Manual 143
10 Parts for Maintenance Accessory Kit, Bio-inert (G5611-68755) Accessory Kit, Bio-inert (G5611-68755) Accessory Kit (Bio-inert) (p/n G5611-68755) 144 p/n Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m G5611-60502 Capillary 900 x 0.17 mm, titanium (Bio-inert) pump to thermostatted autosampler 5042-9954 Tubing clip (2x), re-order 4/pk G5611-60500 Capillary 400 x 0.
Parts for Maintenance Starter-Kit Bio-inert 10 Starter-Kit Bio-inert Starter-Kit Bio-inert HPLC (p/n G5611-68707) p/n Description 9301-1420 (3x) Solvent bottle, transparent 9301-1450 Solvent bottle, amber 01018-22707 PTFE frits (pack of 5) 5182-0716 Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/pk 5182-0717 Blue screw caps 100/pk 5063-6507 (2x) Chip, Column I.D. Assy 5041-2168 (2x) Solvent inlet filter, 20 µm pore size G5611-68710 Capillary/Fitting Starterkit ID.
10 Parts for Maintenance Solvent Cabinet Solvent Cabinet Item p/n Description 1 5065-9981 Solvent cabinet, including all plastic parts 2 5043-0207 Name plate 1260 3 5065-9954 Front panel, solvent cabinet 4 5042-8567 Leak pan 5 9301-1420 Solvent bottle, transparent 6 9301-1450 Solvent bottle, amber 7 G1311-60003 Bottle-head assembly ' & ( 146 ) Bio-inert Quaternary Pump User Manual
Parts for Maintenance Solvent Cabinet 10 , *$+ Figure 15 Solvent Cabinet Parts Bio-inert Quaternary Pump User Manual 147
10 Parts for Maintenance Bottle Head Assembly Bottle Head Assembly Item p/n Description G1311-60003 Bottle-head assembly 1 5063-6598 Ferrules with lock ring (10/Pk) 2 5063-6599 Tube screw (10/Pk) 3 Wire marker 4 5062-2483 Solvent tubing, 5 m 5 5062-8517 Inlet filter adapter (4/Pk) 6 5041-2168 Solvent inlet filter, 20 µm pore size ( ) ' & * + Figure 16 148 Bottle-Head Assembly Parts Bio-inert Quaternary Pump User Manual
Parts for Maintenance Hydraulic Path 10 Hydraulic Path For bio-inert modules use bio-inert parts only! Item p/n Description 1 G5611-60500 Capillary 400 x 0.17 mm, titanium (Bio-inert) pump to injector 1 G5611-60502 Capillary 900 x 0.
10 Parts for Maintenance Hydraulic Path ' ( ) & * + Figure 17 150 Hydraulic Flow Path Bio-inert Quaternary Pump User Manual
Parts for Maintenance System Tool Kit 10 System Tool Kit HPLC System Tool Kit (p/n G4203-68708) p/n Description 0100-1681 Adapter syringe/seal wash tube 0100-1710 Mounting Tool for Tubing Connections 01018-23702 Insert tool 5023-0240 Hex driver, ¼", slitted 8710-0060 Hex-key wrench, 9/64 inch 8710-0510 (2x) Wrench open 1/4 — 5/16 inch 8710-0641 Hex key set 1 – 5 mm 8710-0899 Pozidriv screwdriver 8710-1534 Wrench, 4 mm both ends, open end 8710-1924 Wrench open 14 mm 8710-2392 Hex ke
10 Parts for Maintenance Preventive Maintenance Kit Preventive Maintenance Kit For bio-inert modules use bio-inert parts only! 152 p/n Description G5611-21503 (2x) Piston Seal PTFE (Bio-inert) 01018-22707 PTFE frits (pack of 5) 5067-4728 Seal cap 0905-1731 (2x) Wash Seal PTFE for Bio-inert Pump 600 bar 01018-07102 (2x) Gasket (Seal wash) 5042-8507 Seal wash pump cartridge (silicone tubing) Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 11 Identifying Cables Cable Overview 154 Analog Cables 156 Remote Cables 158 BCD Cables 161 CAN/LAN Cables 163 External Contact Cable Agilent Module to PC 164 165 Agilent 1200 Module to Printer 166 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.
11 Identifying Cables Cable Overview Cable Overview NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Identifying Cables Cable Overview 11 CAN cables p/n Description 5181-1516 CAN cable, Agilent module to module, 0.
11 Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made.
Identifying Cables Analog Cables 11 Agilent Module to General Purpose p/n 01046-60105 Pin Pin Agilent module 1 Bio-inert Quaternary Pump User Manual Signal Name Not connected 2 Black Analog - 3 Red Analog + 157
11 Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.
Identifying Cables Remote Cables 11 Agilent Module to 3396 Series III / 3395B Integrators p/n 03396-61010 Pin 33XX Pin Agilent module Signal Name 9 1 - White Digital ground NC 2 - Brown Prepare run Low 3 3 - Gray Start Low NC 4 - Blue Shut down Low NC 5 - Pink Not connected NC 6 - Yellow Power on High 14 7 - Red Ready High 4 8 - Green Stop Low NC 9 - Black Start request Low 13, 15 Active (TTL) Not connected Agilent Module to Agilent 35900 A/D Converters p/n 5061-33
11 Identifying Cables Remote Cables Agilent Module to General Purpose p/n 01046-60201 160 Pin Universal Pin Agilent module Signal Name Active (TTL) 1 - White Digital ground 2 - Brown Prepare run Low 3 - Gray Start Low 4 - Blue Shut down Low 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop Low 9 - Black Start request Low Bio-inert Quaternary Pump User Manual
11 Identifying Cables BCD Cables BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules.
11 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 162 Pin 3396 Pin Agilent module Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 +5V Low Bio-inert Quaternary Pump User Manual
Identifying Cables CAN/LAN Cables 11 CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables p/n Description 5181-1516 CAN cable, Agilent module to module, 0.
11 Identifying Cables External Contact Cable External Contact Cable 5 10 15 1 6 11 One end of this cable provides a 15-pin plug to be connected to Agilent modules interface board. The other end is for general purpose.
Identifying Cables Agilent Module to PC 11 Agilent Module to PC p/n Description G1530-60600 RS-232 cable, 2 m RS232-61600 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
11 Identifying Cables Agilent 1200 Module to Printer Agilent 1200 Module to Printer 166 p/n Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module.
Bio-inert Quaternary Pump User Manual 12 Hardware Information Electrical Connections 168 Rear View of the Module 169 Interfaces 170 Overview Interfaces 172 Setting the 8-bit Configuration Switch (without On-board) LAN Communication Settings for RS-232C 177 Special Settings 179 176 This chapter describes the pump in more detail on hardware and electronics.
12 Hardware Information Electrical Connections Electrical Connections • The CAN bus is a serial bus with high speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization. • One analog output provides signals for integrators or data handling systems. • The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shut down, prepare, and so on.
Hardware Information Electrical Connections 12 Rear View of the Module Figure 18 Rear view of the pump Bio-inert Quaternary Pump User Manual 169
12 Hardware Information Interfaces Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 9 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio-inert Quat Pump 2 Yes No Yes 1 Yes G4220A/B Bin Pump 2 No Yes Yes No Yes G1361A Prep Pump 2 Yes No Yes
Hardware Information Interfaces Table 9 12 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G1314B VWD VL G1314C VWD VL+ 2 Yes No Yes 1 Yes G1314E/F VWD 2 No Yes Yes 1 Yes G4212A/B DAD 2 No Yes Yes 1 Yes G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL 2 No Yes Yes 2 Yes G1321B FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO G1170A Valve Driv
12 Hardware Information Interfaces NOTE The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via LAN. The inter-module communication is done via CAN. • CAN connectors as interface to other modules • LAN connector as interface to the control software • RS-232C as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Overview Interfaces CAN The CAN is inter-module communication interface.
Hardware Information Interfaces NOTE 12 There is no configuration possible on main boards with on-board LAN. These are pre-configured for • 19200 baud, • 8 data bit with no parity and • one start bit and one stop bit are always used (not selectable). The RS-232C is designed as DCE (data communication equipment) with a 9-pin male SUB-D type connector.
12 Hardware Information Interfaces >chigjbZci BVaZ Figure 19 E8 ;ZbVaZ ;ZbVaZ BVaZ RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on.
12 Hardware Information Interfaces • standard TTL levels (0 V is logic true, + 5.0 V is false), • fan-out is 10, • input load is 2.2 kOhm against + 5.0 V, and • output are open collector type, inputs/outputs (wired- or technique). NOTE All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with respect to the ground terminal).
12 Hardware Information Setting the 8-bit Configuration Switch (without On-board) LAN Setting the 8-bit Configuration Switch (without On-board) LAN The 8-bit configuration switch is located at the rear of the module. Modules that do not have their own LAN interface (e.g. the TCC) can be controlled through the LAN interface of another module and a CAN connection to that module.
Hardware Information Setting the 8-bit Configuration Switch (without On-board) LAN NOTE The following tables represent the configuration switch settings for the modules without on-board LAN only. Table 12 NOTE 12 8-bit Configuration Switch (without on-board LAN) Mode Select 1 2 RS-232C 0 1 Reserved 1 0 TEST/BOOT 1 1 3 4 5 Baudrate 6 7 Data Bits 8 Parity Reserved RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369A/B.
12 Hardware Information Setting the 8-bit Configuration Switch (without On-board) LAN Table 14 Baudrate Settings (without on-board LAN) Switches Baud Rate 3 4 5 0 0 0 0 0 0 0 Table 15 Switches Baud Rate 3 4 5 9600 1 0 0 9600 1 1200 1 0 1 14400 1 0 2400 1 1 0 19200 1 1 4800 1 1 1 38400 Data Bit Settings (without on-board LAN) Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 16 Parity Settings (without on-board LAN) Switches Parity
Hardware Information Setting the 8-bit Configuration Switch (without On-board) LAN 12 Special Settings The special settings are required for specific actions (normally in a service case). Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part). If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident mode. It is not operable as a module.
12 Hardware Information Setting the 8-bit Configuration Switch (without On-board) LAN 180 Bio-inert Quaternary Pump User Manual
Bio-inert Quaternary Pump User Manual 13 Appendix General Safety Information 182 The Waste Electrical and Electronic Equipment Directive Batteries Information Radio Interference Sound Emission 185 186 187 188 Agilent Technologies on Internet 189 This chapter provides addition information on safety, legal and web.
13 Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements.
Appendix General Safety Information 13 the instrument must be made inoperative and be secured against any intended operation. Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed.
13 Appendix General Safety Information Safety Symbols Table 19 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages. Indicates a protected ground terminal. Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product.
Appendix The Waste Electrical and Electronic Equipment Directive 13 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005. NOTE This product complies with the WEEE Directive (2002/96/EC) marking requirements.
13 Appendix Batteries Information Batteries Information WA R N I N G Lithium batteries may not be disposed-off into the domestic waste. Transportation of discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Danger of explosion if battery is incorrectly replaced. ➔ Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries.
Appendix Radio Interference 13 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB.
Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download.
Index Index 8 8-bit configuration switch without On-Board LAN 176 A accessory kit 29, 144 active inlet valve cartridge 114 active inlet valve 114, 142 active seal wash 10, 58 adapter 114 Agilent Diagnostic software 69 Agilent Lab Advisor software 69 Agilent Lab Advisor 69 Agilent on internet 189 algae growth 49 alternative seal material 59 ambient non-operating temperature 25 ambient operating temperature 25 analog signal 174 analog cable 156 apg remote 174 AUTO mode 16 B ball-screw drive 13 battery sa
Index error messages pump error 80 error messages compensation sensor open 78 compensation sensor short 78 encoder missing 87 fan failed 79 ignition without cover 79, 79 index adjustment 90 index limit 90 index missing 91 initialization failed 92 inlet valve fuse 84 inlet-valve missing 87 leak sensor open 77 leak sensor short 77 leak 76 missing pressure reading 83 motor drive power 86 pressure above upper limit 81 pressure below lower limit 82 pump configuration 83 pump head missing 89 remote timeout 75 se
Index N non-operating altitude 25 non-operating temperature 25 O operating Altitude 25 operating temperature 25 operational hints, MCGV 57 optimization stack configuration 30 optional interface board 134 outlet valve 117, 140 overview, pump 11 P packaging damaged 28 parts identification cables 153 parts accessory kit 144 active inlet valve 142 bottle head assembly 148 damaged 28 hydraulic path 149 missing 28 outlet valve 140 pump head assembly 138 purge valve 141 solvent cabinet 146 starter kit 145 syste
Index system pressure test, evaluating results 99 system pressure test 65, 97 running the test 99 system setup and installation optimizing stack configuration system tool kit 151 WEEE directive weight 25 185 30 T temperature limit exceeded 88 temperature out of range 88 temperature sensor 76 test functions 64 test function introduction 96 timeout 74 troubleshooting error messages 73, 64 status indicators 64, 66 U unpacking the pump user interfaces 68 28 V vacuum degasser 10, 39 valve failed 85 valve
www.agilent.com In This Book This manual contains technical information about the Agilent 1260 Infinity Bio-inert Quaternary Pump G5611A. The manual describes the following: • introduction, • site requirements and specifications, • installing the pump, • using the pump, • optimizing performance, • troubleshooting and diagnostics, • maintenance, • parts and materials for maintenance, • identifiying cables, • hardware information, • appendix.
