User manual

Bio-inert Quaternary Pump User Manual 45
Installing the Pump
3
Priming the System
Tab le 4 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation
When switching between
reverse phase and normal
phase (both times)
Isopropanol
Isopropanol
Best solvent to flush air out of
the system
Miscible with almost all
solvents
After an installation Ethanol or methanol Alternative to isopropanol
(second choice) if no
isopropanol is available
To clean the system when
using buffers
After changing aqueous
solvents
HPLC grade water
HPLC grade water
Best solvent to re-dissolve
buffer crystals
Best solvent to re-dissolve
buffer crystals
After the installation of normal
phase seals (P/N 0905-1420)
Hexane + 5% isopropanol Good wetting properties