User manual

56 Bio-inert Quaternary Pump User Manual
5 Optimizing Performance
When to Use a Degasser
When to Use a Degasser
The bio-inert quaternary pump has a built-in degasser, which should always be
included to the flow path.
Operational Hints for the Internal Degasser
If you are using the degasser for the first time, if the degasser was switched off
for some time (for example, overnight), or if the degasser lines are empty, you
should prime the degasser before running an analysis.
The degasser can be primed either by drawing solvent through the degasser
with a syringe or by pumping with the pump.
Priming the degasser with a syringe is recommended, when:
• degasser is used for the first time, or solvent tubes are empty, or
• changing to solvents that are immiscible with the solvent currently in the
vacuum tubes.
Priming the degasser by using the pump at high flow rate (3 – 5 mL/min) is
recommended, when:
• pump was turned off for some time (for example, during night) and volatile
solvent mixtures are used, or
• solvents have been changed.