User manual

114 1260 Nanoflow Pump User Manual
8 Test Functions and Calibration
Normal Mode Pressure Test
Running the Pressure Test
Running the test from the Agilent Lab Advisor
1 Select the Pressure Test from the Test Selection menu.
2 Start the test and follow the instructions.
“Evaluating the Results” on page 115 describes the evaluation and
interpretation of the Pressure Test results.
For detailed instructions refer to the Agilent Lab Advisor Software.
When • If problems with small leaks are suspected
• After maintenance of flow-path components (e.g. pump seals, injection seal) to prove pressure
tightness up to 400 bar bar.
Tools required Wrench 1/4 inch
Parts required # p/n Description
1 01080-83202 Blank nut
1 500 ml Isopropanol
Preparations • Place a bottle of LC-grade isopropanol in the solvent cabinet and connect it to channel A2.
• Install the manual purge valve from the accessories kit to pump head A.
• Move the inline filter outlet capillary from the EMPV to to manual purge valve.
NOTE
Make absolutely sure that all parts of the flow path that are part of the test are very
thoroughly flushed with isopropanol before starting to pressurize the system! Any trace of
other solvents or the smallest air bubble inside the flow path definitely will cause the test
to fail!
NOTE
Make sure to release the pressure by slowly opening the purge valve when the test has
finished.