User manual

1260 Nanoflow Pump User Manual 123
Test Functions and Calibration
8
Leak Test
Fourth plateau negative or unstable and at least one other plateau
positive
Probable cause Suggested actions
1 Air in pump chamber of channel B or seals
not yet seated.
Flush channel B thoroughly with isopropanol
under pressure (restriction capillary).
2 Loose active inlet valve in channel B.
Tighten the active inlet valve in channel B
(14mm wrench). Do not overtighten!
3 Loose pump head screws in channel B.
Ensure the pump head screws in channel B are
tight.
4 Loose outlet valve in channel B.
Ensure the sieve in the outlet valve is installed
correctly. Tighten the outlet valve.
5 Leaking seal or scratched piston in channel
B1.
Exchange the pump seals in channel B. Check
the pistons for scratches. Exchange if scratched.
6 Defective active inlet valve in channel B.
Exchange the active inlet valve in channel B.