User manual

1260 Nanoflow Pump User Manual 125

Test Functions and Calibration

EMPV Cleaning

1260 Nanoflow Pump EMPV Cleaning Description

Depending on the application, particles can sometimes collect in the EMPV.

This fast cleaning routine is designed to remove such particle deposits. The

routine should always be performed when the EMPV is suspected of being

leaky or contaminated with particles.

The outlet of the EMPV is plugged with an SST blank nut. After a short

flushing routine the EMPV is closed and the pressure is increased to

approximately 380 bar. The EMPV is then opened and the pressure is released

very quickly. This procedure is repeated several times in a sequence.

Running the Test

1 Fill vacuum degasser channel A1 and B1 with solvents (the test requires

filled vacuum chambers). We recommend that you use channel A with

aqueous solvent. If you use a different channel, you must ensure

• the miscibility of the solvent

• that no precipitation of buffer occurs

2 Plug the pump outlet with blank nut at EMPV outlet.

3 Disconnect the EMPV to flow sensor at the EMPV outlet. Plug the EMPV

outlet port with blank nut (01080-83202).

4 Execute test.

5 Check result with Pressure Test if necessary.

6 Remove the blank nut.

7 Reconnect the EMPV to flow sensor capillary. Do not overtighten!