User manual

1260 Nanoflow Pump User Manual 59
Installing the Module
3
Get the System Ready for the First Injection
7 Repeat steps 4 to 6 for the three remaining solvent channels.
8 When all 4 channels are manually primed, remove the paper towel from the
pump leak tray. Make sure that the pump leak sensor is dry before turning
on the pump.
Priming Your System With the Pump
1 At the pump, activate the Purge Mode and set the flow rate to 2.5 ml/min.
2 Flush the vacuum degasser and all tubes with at least 5 ml of solvent.
3 Set flow to required value of your application and activate the pump micro
mode.
4 Pump for approximately 5 minutes before starting your application.
5 Repeat step 1 on page 59 through step 2 on page 59 for the other channel(s)
of the pump.
WARNING
When opening capillary or tube fittings solvents may leak out.
The handling of toxic and hazardous solvents and reagents can bear health risks.
➔ Please observe appropriate safety procedures (for example, goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
NOTE
When the pumping system has been turned off for a certain time (for example, overnight)
oxygen will re-diffuse into the solvent channel between the vacuum degasser and the
pump. Solvents containing volatile ingredients will slightly lose these, if left in the degasser
without flow for a prolonged period of time. Therefore purging each channel at 2.5 ml/min
for 1 minute is required before starting an application.