User manual

62 1260 Nanoflow Pump User Manual
4 Using the Pump
Hints For Successfully Using the Pump
Hints For Successfully Using the Pump
Pump Issues
• Flush the pump extensively. First with in the Purge Mode, second with a
pressure applied to remove all the gas bubbles. It is recommended to do this
first with 100 % A and than 100 % B.
• The system pressure must be higher than 20 bar at the pump outlet.
• In Micro Mode, unexpected high column flow variation is an indication for
dirt within the system, blocked frits or leaking pump valves.
• Always place the solvent cabinet with the solvent bottles on top (or at a
higher level) of the pump.
• Make sure the pump is only used with the provided stainless steel solvent
filters (Solvent inlet filter, stainless steel (p/n 01018-60025)). Glas inlet
filters may release particles that impact the operation of the EMPV
(electromagnetic proportioning valve). For the same reason, never use the
pump without filters.
• Use the provided brown solvent bottle for aqueous solvents as the lower
light transmission will help to prevent the growth of algae. Clean the bottle
regularly (e.g. every second day) and discard any unused solvent.
• When using buffer solutions, flush the system with water before switching
it off.
• Check the pump pistons for scratches when changing the piston seals.
Scratched pistons will lead to micro leaks and will decrease the lifetime of
the seal.
• After changing the piston seals apply the seal wear-in procedure.
• Place the aqueous solvent in channel A and the organic solvent in
channel B. The default compressibility and flow sensor calibration settings
are set accordingly. Always use the correct calibration values.
• When running in Micro Mode, check the correct instrument setup (flow
sensor type, used mixer and filter).