User manual

1260 Nanoflow Pump User Manual 63
Using the Pump
4
Hints For Successfully Using the Pump
Fused Silica Capillary Issues
• When connecting a capillary (especially at the column) press it smoothly
into the fitting to avoid void volumes. Incorrect setting will result in
dispersion, causing tailing or footing peaks.
• Be careful when bending fused silica capillaries. The diameter must not be
smaller than 40 mm.
• When you replace a part, especially a capillary, clean it with acetone.
• If a fused silica capillary leaks, do not retighten the fitting under flow. Set
the column flow to zero, re-insert the capillary, tighten and set the new
column flow.
• Avoid the use of alkaline solutions (pH > 8.5 ) as they attack the fused silica
of the capillaries.
• Be careful not to crush capillaries when closing module doors.
• A broken capillary can release silica particles into the system (e.g. cell)
causing problems in the system downstream of the crack.
• Often, a clogged capillary can be recovered by backflushing with acetone.
NOTE
The quartz core of PEEK/fused silica capillaries will crack and debris will clog the flow path
if the fittings are overtightened. Fittings shouldn't be tightened harder than finger tight plus
1/4 turn with a wrench.
)%bb