User manual

1260 Nanoflow Pump User Manual 67

Using the Pump

Checkout procedure for a G2229A Nano LC System

Use this procedure to confirm that

• the system has been installed correctly

• the Nanoflow LC System performs within specification

• a technical problem is caused by the Nanoflow LC System

1 Purge channel A1 with 100 % water at 2.5 mL/min for 2 min

2 Purge channel B1 with 100 % aetonitrile at 2.5 mL/min for 2 min.

3 Pump 10 µL/min, normal mode, 100 % (water) for at least 5 min.

4 Pump 10 µL/min, normal mode, 100 % B (acetonitrile) for at least 5 min.

5 Check the pressure tightness of the system by executing a micro pressure

test with port 6 of the autosampler valve blanked off.

6 Install the restriction capillary to port 6 of the autosampler injection valve.

7 Pump 1.5 µL/min, micro mode, 70 % A (water) / 30 % B (acetonitrile). Pump

as long as it takes for the pressure to get stable. Continue pumping for at

least 5 min more before continuing.

Parts required # Description

G1379B 1260 Micro Degasser

G2226A 1260 Nanoflow Pump

G1377A 1260 Micro High Performance Autosampler

G2226-67300 Nanoflow restriction capillary

Preparations • Channel A1: Water

• Channel B1: Acetonitrile

• Channel A2: Isopropanol

NOTE

You can speed up the preparation by skipping steps 3 and 4 and running step 7 in pressure

control mode at 140 bar (pressure control mode can be enabled in the module service

center of Lab Advisor software).