User manual

68 1260 Nanoflow Pump User Manual
4 Using the Pump
Checkout procedure for a G2229A Nano LC System
8 Pump 0.6 µL/min, micro mode, 70 % A (water) / 30 % B (acetonitrile). Pump
as long as it takes for the pressure to become stable. Pump at least 5 min
more before continuing.
Method Parameters
Method Parameters Nanoflow Pump
NOTE
Make absolutely sure that all parts of the flow path have been thoroughly flushed before
starting the checkout procedure. Any trace of other solvents, air bubbles or leaks in the
flow path will negatively affect the results.
Tabl e 16 Method Parameters for Nanoflow Pump
Parameter Value
Column flow 0.6 µL/min
Stoptime 15 min
Solvent A 70 %
Solvent B 30 %
Calibrated as H
2
O/ACN
Primary flow 200 – 500 µL/min
Compressibility A
50·10
-6
/bar
Compressibility B
115·10
-6
/bar
Min stroke A and B Auto
Fast composition change ON