User manual

70 1260 Nanoflow Pump User Manual
4 Using the Pump
Checkout procedure for a G2229A Nano LC System
Test Results and Evaluation
Typical pressure in bypass mode at 600 nL/min is 100 bar and at
300 nL/min50 bar (with the Fused Silica/PEEK capillary 25 µm, 35 cm (p/n
G1375-87322) installed between the flow sensor and port 1 of the injection
valve plus the restriction capillary installed on port 6 of the injection valve).
The evaluation is done by a visual inspection of the test results: The average
pressure between the different plateaus at the same flow rate must be in a
range of ±2 bar and typically ±1 %. The pressure at 300 nL/min should be half
of the pressure at 600 nL/min.
If the test results are not in the expected range verify that the flow path has
been thoroughly flushed and is filled with homogenous solvent composition.
The flow rate for a nano pump is very low compared to the volume of the flow
path. Depending on the configuration, it may take several hours to obtain
stable conditions.
NOTE
Due to capillary ID tolerances the nominal pressure at 600 nL/min and 300 nL/min might
be different from system to system. These differences can be up to ± 40 %.
NOTE
If no UV detector is connected to the system you will not be able to open your test data file
to review the pressure and the flow profile acquired during the run. In this case, locate and
copy the signal file DAD1A.CH from the directory HPCHEM/1/DATA/DEMO/ISOCRA.D to
the directory for your checkout test data file before opening the file for review.