Opsys MW™ Microplate Washer User’s Guide IMPORTANT Please read carefully before using the Opsys MW
Part No. 91000051, Revision B This manual is published by DYNEX Technologies, Inc. Questions or comments regarding the content of this manual can be directed to the address below or to your DYNEX Technologies representative. DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 Tel. USA (703) 631-7800 (800) 288-2354 Fax. (703) 803-1441 TM OPSYS MW is a trademark of DYNEX Technologies, Inc.
Warranty and Special Provisions Limited Warranty DYNEX Technologies products are fully guaranteed for one year against defects in parts, materials, and workmanship.
Table of Contents Table of Contents About this Manual iii Chapter 1 Introduction Description 1 1 Programming Wash Head Wash Head Positions Wash Fluid Waste 2 3 4 5 5 Features Specifications Warning Labels 7 9 11 Chapter 2 Installation Unpacking 13 13 Materials Provided Hardware Components Positioning the Instrument Installing the Wash Head Installing the Wash Container Installing the Waste Container Turning on the System Chapter 3 Configuration The Keypad Menus The SETUP Menu The PROGRAM Menu C
Table of Contents Cleaning and Decontamination Cleaning the Containers Replacing the Tubing Pump Calibration 69 70 71 74 Chapter 7 Troubleshooting Operational Problems 77 77 Chapter 8 Service Service Procedures Spare Parts Returning a Washer for Service Contact DYNEX Technologies 81 81 82 83 85 Index 86 ii Opsys MW™ User’s Guide
About this Manual About this Manual This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system. This manual gives you the information needed to: • Review safety precautions. • Install the Opsys MW™ system. • Understand the Opsys MW™ menus. • Set the Opsys MW™ system up to suit your exact needs. • Create or modify wash protocols. • Run wash protocols using the Opsys MW™ system. • Perform required preventive maintenance.
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Description Chapter 1 Introduction The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so that all of the columns (or rows) are washed in the same manner, or different wash cycles can be applied to specified columns (or rows) on the plate.
Description Programming All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system. Note: Memory limitations may restrict the size and complexity of an individual wash protocol. In addition, the system can be configured with up to ten different plate types so that the wash head positions for each plate type can be specified.
Description Wash Head The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins. Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously. The following wash heads can be fitted on the Opsys MW™: Wash Head Description 1x8 Eight sets of dual wash pins for washing a 96-well microplate one column (8 wells) at a time.
Description Wash Head Positions The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm. Note: Procedures for specifying wash head positions for various plate types are described on page 30. 4 Wash Head Position Description Dispense Height Positions the aspiration pins slightly below the top of the well so that fluid can be dispensed into the well up to a certain level before it is aspirated.
Description Wash Fluid A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray during a Purge operation.
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Features Features The Opsys MW™ system has a number of performance and convenience features.
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Specifications Specifications Dimensions and Weight Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.
Specifications Input Ranges Assay Programming Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment: 1) Aspirate: Cycles 1 - 9 (Increment: 1) Dispense 50 - 999 (Increment: 1 µL) Purge 50 - 9999 (Increment: 1 µL) Fill 50 - 300 (Increment: 1 µL) Soak 1 - 999 (Increment: 1 second) Plate Setup
Warning Labels Warning Labels The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below. Label Description Alternating current is present. (English) Caution symbol. Refer to the Routine Maintenance chapter. (French) Attention (voir documents d’accompagnement). (English) Caution, hot surface. (French) Attention, surface chaude.
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Unpacking Chapter 2 Installation Unpacking Materials Provided The main carton contains the instrument. The contents of this carton are listed below: Article Quantity Opsys MW™ System 1 Instrument Power Cable 1 Cleaning Wire, Aspirate 1 Cleaning Wire, Dispense 1 Instrument Configuration Report 1 User’s Guide 1 Declaration of Conformity 1 Wash Head, 1 x 8 or 1 x12 1 Plate Holder 1 A sub-carton contains the wash container and waste container assemblies.
Unpacking To unpack the components: 1. Place the Opsys MW™ instrument in the position where it will be located for use. 2. Place the Wash Container, Waste Container and Foam/Aerosol Trap at the rear of the instrument. 3. Examine the packaging to be sure that the power cord and other materials have been removed. Please save packaging material for future use. 4. Verify that all of the materials listed above have been unpacked. 5. Inspect the components for damage.
Hardware Components Hardware Components Hardware components of the Opsys MW™ instrument are shown in Figure 2 and described below. Note: The Wash Container, Waste Container and Foam/Aerosol Trap are external to the instrument and are not shown in Figure 2. Wash Head ® Microtiter Plate Plate Carrier Display Panel Keypad Figure 2. The Opsys MW™ System • Wash Head. The wash head contains sets (1 x 8 or 1 x 12) of dual wash pins.
Hardware Components 16 • Keypad. The keypad is used by the operator to select commands and enter wash parameters. • RS232 Interface. The system includes an RS232 interface so that an external computer can be connected for technical service purposes. • Wash Container. (Shown in Figure 1). The wash container contains the wash fluid that is used for Dispense, Fill and Purge operations. • Waste Container. (Shown in Figure 1). The waste container contains the fluid that is aspirated from the wells.
Hardware Components Connectors at the rear of the Opsys MW™ system are shown in Figure 3. Connections for the power cord, dispense pump power, waste bottle level sensor, waste bottle vacuum line and an external computer are made at the rear of the system. Important: Be sure that the vacuum exhaust is not obstructed. Figure 3.
Positioning the Instrument Positioning the Instrument Determine the area where the system will be located. You will need a firm and level area that is approximately 51 cm (20 inches) wide, 62 cm (24 inches) deep, and 40 cm (16 inches) high for the Opsys MW™.
Installing the Wash Head Installing the Wash Head The Wash Head must be located on the Wash Head Arm. To install the Wash Head: 1. Position the Arm over the Purge Reservoir portion of the Plate Holder. 2. Place the Wash Head on the two Arm Pins, being careful to properly seat the Wash Head on the support pins. 3. Level the Wash Head in relation to the Plate Holder and tighten the two thumbscrews at the front of the Wash Head / Arm Assembly.
Installing the Wash Container Installing the Wash Container The dispense tubing and dispense pump power cable must be connected. To install the Wash Container: 1. Fill the Wash Container with the wash solution that is to be used. Note: The Wash Container contains up to 4 liters. 2. Insert the Pick-Up Filter into the Wash Container and secure the cap. Attach the Quick-Connector at the end of the pump tubing, to the mating connector in the cap. 3.
Installing the Waste Container Installing the Waste Container To install the Waste Container and Foam/Aerosol Trap: 1. Insert the Quick-Connect end of the vacuum tubing on the Foam/Aerosol Trap to the Quick-Connect vacuum connector at the rear of the instrument Figure 3). 2. Screw on the Waste Container cap. 3. Route the waste tubing through the lower slot at the front corner of the instrument and through the routing clips at the front of the instrument. 4.
Turning on the System Turning on the System Note: Depending upon local electrical codes, an uninterruptible power system (UPS) may be required in your laboratory. A UPS is not provided with the Opsys MW™ system. 1. Connect the Opsys MW™ to the laboratory electrical supply outlet. CAUTION: The Opsys MW™ instrument must be connected to properly grounded electrical outlets. Obtain assistance from a qualified electrician to verify that your electrical outlets are properly grounded.
The Keypad Chapter 3 Configuration The Keypad The keypad is used to access the menus and commands for setting up the Opsys MW™ entering new wash protocols and running the wash protocols. The keypad contains scroll keys, function keys, and alphanumeric keys. The keys on the keypad are shown in Figure 4. Scroll Keys Display Panel Function Keys Scroll Keys Alphanumeric Keys 1 ABC 4 JKL 7 STU 2 DEF 5 MNO 8 VWX 3 GHI 6 PQR 9 YZ ENTER DEL START +-*/= 0 <>_() CANCEL Figure 4.
The Keypad The use of each of the keys is summarized below: Key Purpose 24 Scroll keys The functionality of the scroll keys depends upon what is being displayed. During display of the main menu (page 26), pressing the up or down scroll keys will display successive wash protocol numbers and names (or dashes, if a protocol is not yet named). Horizontal scrolls will scroll through selections for the selected menu. Repeatedly pressing the horizontal scroll button will show the next two items in a list.
The Keypad Key Alphanumeric Keys Purpose The numeral shown on the key is displayed by pressing the key once. If the key is pressed two to seven times rapidly, one of the letters on the key will be displayed. The letter that is displayed depends upon the number of times the key is pressed.
Menus Menus The Opsys MW™ menus are hierarchical and are organised by function. There are two main menus: Setup and Program. Menu Purpose SETUP Configure the Instrument PROGRAM Create or edit plate washing protocols * * A protocol includes general information (e.g. the type of microplate to be washed) as well as a listing of the various steps to be used in the washing process. Once the instrument is switched on and the self-test is successfully completed, the main menu is displayed.
Menus The SETUP Menu Press the function key under SETUP to display the SETUP menu (Figure 5). The SETUP menu contains commands to configure the instrument. 1: TESTNAME SETUP PROGRAM Select Option HEAD PLATE Select Option VERSION CAL. Figure 5. SETUP Menu The PROGRAM Menu Press the function key under PROGRAM to display the PROGRAM menu (Figure 6). The PROGRAM menu contains commands to name and create a new wash protocol or to edit an existing wash protocol.
Configuring the System Configuring the System The Opsys MW™ must be configured before it is operated for the first time. The commands for doing this are contained in the SETUP menu (Figure 7). Use ARROW keys to scroll through the menus. 1: TESTNAME SETUP PROGRAM Select Option HEAD PLATE Select Option VERSION CAL. Figure 7. SETUP Menu The purpose of each command on the SETUP menu is summarised below: 28 Command Purpose HEAD Specify the type of head that is fitted PLATE Displays the PLATE menu.
Configuring the System Specifying the Wash Head The HEAD command allows the installed wash head to be specified: 1. Select SETUP. The display will read: Select option HEAD PLATE 2. Select HEAD. The current wash head selection is displayed in the upper line: Wash head? 8 8 If needed, scroll right to view the remaining selection: Wash head? 12 8 3. Select the desired option using the function key.
Configuring the System Specifying Plate Parameters The PLATE command allows plate parameters to be specified for up to 10 plate types. When setting plate parameters, a default value is displayed for each of the wash head positions. The default values are factory-set and represent approximate starting values for each wash head position. Note: The wash head positions are described on page 4. Increasing the value of a wash head position by one unit lowers the head 0.
Configuring the System To specify parameters for a plate type: 1. Obtain an empty plate of the plate type being specified and place it in the plate holder. 2. Select SETUP (if needed). The display will read: Select option HEAD PLATE 3. Select PLATE. The name of the first plate is displayed in the lower line: Select plate 1: - - - - - - - - - - Note: Dashes will be displayed if the plate has not yet been named. 4.
Configuring the System 7. Enter the dispense height using the keypad and press ENTER. The display will read: Well top? 160 SHOW Note: The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed. 8. Enter the desired value using the keypad and press ENTER. The display will read: ASP. HEIGHT? SHOW 380 Note: The default value is displayed.
Configuring the System Remember if your protocol requires sweeping, that must first be specified in the plate definition used in the protocol. 11. Select YES to specify a sweep on all aspiration cycles. The display will read: Sweep height? SHOW 370 Note: The default value is displayed. Select SHOW to move the wash head to the default sweep position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed. 12.
Configuring the System 15. Enter the desired value using the keypad and press ENTER. The display will read: Select option HEAD PLATE Note: If NO is selected for Bottom wash? (Step 13), the display will read as shown above.
Configuring the System Displaying the Software Version The VERSION command displays the software version that is currently installed on the Opsys MW™ instrument: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option VERSION CAL. 3. Select VERSION. The display will momentarily read: Opsys MW. v1.0 Note: The software version that is displayed will depend on the version installed in the instrument.
Configuring the System Specifying the Pump Parameters The CAL. command allows the calibration values for each pump to be specified: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option VERSION CAL. 3. Select the pump pressure (high or low). The display will read: Pump speed? 190 4. Enter the desired value using the keypad and press ENTER. The display will read: Slope? 2.
Configuring the System Selecting Clean The CLEAN command enables the washer to be cleaned automatically at a specified interval. 1. Scroll right to display the next section: Select option CLEAN 2. Select CLEAN. The display will read: Auto Clean? YES NO 3. Select YES if you want periodic automatic cleaning. The display will read: Interval (Hrs) 1 4. Enter the desired interval using the up/down scroll keys and press ENTER. Note: The minimum interval is 1 hour, and the maximum interval is 9 hours.
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Wash Protocol Operations Chapter 4 Wash Protocols Wash Protocol Operations A wash protocol consists of a series of Purge, Move, Soak, Aspirate, Dispense and Fill operations. Purge, Move and Soak can be carried out in any sequence. Aspirate, Dispense and Fill can only be carried out within a Move operation, and there cannot be a Move within a Move.
Wash Protocol Operations Operation Description Dispense Dispenses a specified amount of fluid into the wells after aspirating the contents of the wells (i.e. wherever a DISPENSE step is added to a protocol, the OPSYS MW will automatically aspirate the wells before dispensing more fluid in order to prevent a well overflow). If a bottom wash is specified, the wash head is then lowered to the bottom wash position so that fluid will be aspirated from the bottom of the wells while fluid is being dispensed.
Creating a New Wash Protocol Creating a New Wash Protocol Specifying the Header NOTE: Pressing CANCEL while creating or editing a protocol will bring you back to the main menu without saving your changes. or (or use keypad) Select one of the 40 protocol numbers. ↓ PROGRAM Select PROGRAM from the main menu. ↓ (keypad) Enter a protocol name (up to 9 characters). ↓ (keypad) Enter an optional password.
Creating a New Wash Protocol To create a new wash protocol header: 1. Display the main menu. 1: - - - - - - - - - SETUP PROGRAM 2. Use the up or down scroll keys to display the protocol number (or use the keypad to enter the protocol number) that is to be created: 6: - - - - - - - - - SETUP PROGRAM Note: Dashes will be displayed if a protocol has not yet been named. 3. Select PROGRAM. The name of the wash protocol is displayed in the lower line: Enter name ---------4.
Creating a New Wash Protocol 7. Re-enter the password and press ENTER. The display will read: Select plate 2: DYNEX_1 Note: The plate name that is shown is an example. The actual name will be the plate name that is entered for that plate type during system configuration (see page 30). 8. Use the up and down scroll keys to select the plate type (or enter the number using the keypad) to be used and press ENTER.
Creating a New Wash Protocol 10. Select YES if the total elapsed time for washing any number of strips within a plate is to be constant. The display will read: Select option MOVE PURGE The sequential steps in the wash protocol can now be specified, as outlined on page 45.
Creating a New Wash Protocol Specifying Wash Cycle Operations The final step in creating a wash protocol is to specify individual operations within the wash protocol. An example of a wash protocol is shown in 45. In this example, the dispense wash pins are purged, and then a Dispense and Aspirate are carried out twice on each of the 12 strips (columns) of a 96-well Microtiter® plate.
Creating a New Wash Protocol To specify a Purge: Note: A purge must be performed outside of a Move cycle. 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE 2. Select PURGE. The display will read: Fluid volume? 350 3. Use the keypad to enter the volume of fluid to be dispensed. Note: A purge volume ranging from 50 to 9,999 microliters can be entered. 4. Press ENTER.
Creating a New Wash Protocol To specify a Move cycle: 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE 2. Select MOVE. The display will read: Start strip? 1 3. Using the keypad, enter the strip number where the move cycle should start. 4. Press ENTER. The display will read: End strip? 12 5. Using the keypad, enter the strip number where the move cycle should end. 6. Press ENTER.
Creating a New Wash Protocol To specify an Aspiration (within a Move): Note: An Aspirate or Dispense must be performed within a Move cycle. 1. Select ASPIRATE. The display will read: Cycles? 1 2. Using the keypad, enter the number of aspiration cycles that are to be to be performed within the MOVE cycle. 3. Press ENTER.
Creating a New Wash Protocol To specify a Dispense (within a Move): Note: A Dispense or Aspirate must be performed within a Move cycle. 1. Select DISPENSE. The display will read: Fluid volume? 350 2. Use the keypad to enter the volume of fluid to be dispensed. Note: A volume ranging from 50 to 999 microliters can be entered. 3. Press ENTER.
Creating a New Wash Protocol 6. Press ENTER. The display will read: Cycles? 1 7. Use the keypad to enter the number of bottom wash cycles to be performed and press ENTER.
Creating a New Wash Protocol To specify a Soak (within a Move): Note: A Soak can be performed either within or outside of a Move cycle. The procedure below describes the procedure for specifying a soak within a Move cycle. The procedure for specifying a Soak outside of a Move cycle is described on page 50. 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Select SOAK. The display will read: Duration? 5 3. Enter the desired soak duration using the keypad.
Creating a New Wash Protocol To specify a Fill (within a Move): Note: A Fill must be performed within a Move cycle. 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Select FILL. The display will read: Fluid volume? 50 3. Use the keypad to enter the volume of fluid to be dispensed during well filling. Note: A volume ranging from 50 to 300 microliters can be entered. 4. Press ENTER.
Creating a New Wash Protocol Ending a MOVE Cycle 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Scroll right to display the next selection. The display will read: Select option END LOOP 3. Select END LOOP.
Creating a New Wash Protocol To specify a Soak (outside of a Move): Note: A Soak can be performed either within or outside of a Move cycle. The procedure below describes the procedure for specifying a soak outside of a Move cycle. The procedure for specifying a Soak within a Move cycle is described on page 47. 1. Scroll right to display the next selection. The display will read: Select option SOAK END 2. Select SOAK. The display will read: Duration? 5 3.
Creating a New Wash Protocol To specify the End of the wash protocol: 1. Scroll right to display the next selection. The display will read: Select option SOAK END 2. Select END.
Modifying a Wash Protocol Modifying a Wash Protocol An existing wash protocol can be modified by using many of the same commands that are used to create a wash protocol. To modify a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the wash protocol to be modified (or enter the number using the keypad). The display will read: 4: NAME_04 SETUP PROGRAM 3. Select PROGRAM.
Modifying a Wash Protocol 6. The cursor is positioned under the first character of the name. If the name is to be changed, enter the name using the keypad and press ENTER. The first step of the wash protocol will be displayed: MOVE 1-12 DELETE 1 Cycles EDIT Note: The example above shows the MOVE operation as the first step in the protocol. The actual operation that is shown will depend upon the protocol that is being modified.
Modifying a Wash Protocol To modify a wash operation: 1. Select EDIT while the operation is displayed. The first step in the operation is displayed: Start strip? 1 Cycles Note: The actual step that is displayed depends upon the operation being modified. 2. Modify the operation, using the same procedures as when creating a new wash protocol. MOVE 1-10 DELETE 1 Cycles EDIT Note: In the example above, the End Strip was changed from 12 to 10.
Modifying a Wash Protocol To delete a wash operation: 1. Select DELETE while the operation is displayed. The operation is removed from the wash protocol. Note: If a MOVE operation is deleted, all operations within the MOVE (i.e., Aspirate, Dispense, Soak and Fill) are also removed.
Modifying a Wash Protocol To insert a wash operation: 1. Scroll right while the operation that the new operation is to precede is displayed: MOVE 1-10 INSERT 1 Cycles Note: The inserted operation will take place immediately before the operation that is displayed. 2. Select INSERT. The display will read: Select option MOVE PURGE 3. Select the option that is to be inserted, using the same procedure as when creating a new wash protocol.
Copying a Wash Protocol Copying a Wash Protocol An existing wash protocol can be copied to another program number. If a wash protocol is already in that location, it will be overwritten. To copy a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the wash protocol to be modified (or enter the protocol number using the keypad). The display will read: 4: NAME_04 SETUP PROGRAM 3. Select PROGRAM.
Copying a Wash Protocol 7. Using the keypad, enter the number of the wash protocol that the commands are to be copied to. If a wash protocol already exists at that location the display will read: Overwrite test? YES NO NO 8. Select YES.
Preparation Chapter 5 Washing a Plate Once the parameters for at least one plate type and one wash protocol have been set, the system can be used to wash a plate. Preparation The only preparation that is required is to check the fluid levels in the Wash Container and the Waste Container. Service the containers if necessary. To empty the waste container: 1. Unscrew the cap of the Waste Container. Lay the cap and level sensor assembly on a paper towel. 2.
Preparation To refill the wash container: 1. Separate the dispense line quick-connector in the cap of the Wash Container. 2. Unscrew the cap of the Wash Container and lay it and the PickUp Filter on a clean paper towel. 3. Fill the Wash Container with the wash fluid that is being used. Note: If the wash solution is being changed, discard the contents of the Wash Container and thoroughly clean it before filling it with the new wash solution. 4.
Washing a Plate Washing a Plate A plate is washed by positioning it on the carrier block, selecting the wash protocol to be run, and pressing the START key. To wash a plate: 1. (If needed) Turn on the instrument. The display will read: 1: TESTNAME SETUP PROGRAM 2. Place the microplate on the plate carrier. Note: Be sure that the plate position on the carrier plate corresponds to the wash head configuration that is being used.
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Routine Maintenance Procedures Chapter 6 Routine Maintenance Routine Maintenance Procedures The Opsys MW™ Microplate Washer is constructed from materials that resist chemical attack. If a spill occurs, it should be cleaned up as soon as possible using a cloth moistened with a mild laboratory detergent (If needed, dilute the laboratory detergent according to the manufacturer’s directions).
Routine Maintenance Procedures • Replace the dispense (Wash) tubing. • Replace the aspirate (Waste) tubing. Note: The dispense tubing and aspiration tubing may need to be replaced more frequently than every six months, depending upon the frequency of use and the severity of operating conditions. Contact DYNEX Technologies for information on replacement tubing. • Replace the in-line hydrophobic filter.
Cleaning and Decontamination Cleaning and Decontamination The Opsys MW™ is constructed from materials that resist chemical attack. Spills should be cleaned as soon as possible. If you need to decontaminate the Opsys MW™ instrument (for example, before servicing the instrument), clean the system and then decontaminate it as described below. CAUTION: Always disconnect the power cable before cleaning the instrument. To clean the system: 1.
Cleaning the Containers Cleaning the Containers Procedures for emptying, cleaning and refilling the Wash Container and the Waste Container are described below. To empty and clean the waste container: 1. Unscrew the cap of the Waste Container. Lay the cap and level sensor assembly on a paper towel. 2. Discard the waste in accordance with local regulations. 3. Rinse the waste container with deionized water. Discard the rinse water.
Replacing the Tubing Replacing the Tubing To replace the dispense tubing: 1. Separate the dispense line quick-connector in the cap of the Wash Container. Unscrew the cap, lay it and the Pick-Up Filter on a clean paper towel. 2. Discard the contents of the Wash Container. Thoroughly rinse the container with deionized water and let it dry. 3. Disconnect and discard the Wash Container Internal Tube and pick-up filter that is attached to the barb on the Quick Connect Fitting in the Cap. 4.
Replacing the Tubing To replace the vacuum tubing: Note: This procedure describes replacement of the vacuum tubing connecting the Waste Container to the Foam/Aerosol Trap. The vacuum tubing connecting the Foam/Aerosol Trap to the rear of the instrument should not require changing. 1. Unscrew the Cap of the Waste Container, lay it and the level sensor assembly on a clean paper towel. 2. Discard the contents of the Waste Container in accordance with local regulations.
Replacing the Tubing To replace the waste tubing: 1. Unscrew the Cap of the Waste Container and lay it with the level sensor assembly on a clean paper towel. 2. Discard the contents of the Waste Container in accordance with local regulations. Thoroughly rinse the container with deionized water and let it dry. 3. Disconnect the waste tubing from the connector on the Waste Container cap and from the waste tubing connector on the wash head. 4. Remove the waste tubing from the instrument. 5.
Pump Calibration Pump Calibration Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three calibration values stored in memory. These values are calculated and stored during manufacture at DYNEX Technologies. When the Opsys MW configuration is changed from one manifold to another, the corresponding calibration settings will be recalled by correctly setting the head size using the SETUP/HEAD feature (refer to page 28 for Specifying the Wash Head).
Pump Calibration 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function (refer to page 36). • A pump speed change of -10 units roughly corresponds to a well volume change of -10µl. 9. If the dispensed volume is too low, increase the pump speed using the SETUP/CAL function (refer to page 36). • A pump speed change of +10 units roughly corresponds to a well volume change of +10µl. 10. Repeat Steps 3-9 until fluid is dispensed with the accuracy required. (e.g.
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Operational Problems Chapter 7 Troubleshooting Operational Problems Problems that may occur during normal operation of the Opsys MW™ are described below. For each problem, the symptom, probable cause and the means for resolving the problem are shown. Symptom Probable Cause Resolution The instrument fails to power up. The cord is not connected. Check that the cord is plugged into the instrument and into the electrical outlet. Verify that the electrical outlet is not controlled by a switch or timer.
Operational Problems Symptom Probable Cause Resolution Liquid is not dispensed from the dispense pins (continued). The wash protocol is incorrectly defined. Check the system configuration and wash protocol for the number fluids and fluid volume. The dispense tubing or the dispense pump power supply is not connected. Verify that the dispense tubing and the dispense power supply are connected (see page 20). The dispense tubing is sealed shut at the dispense valve.
Operational Problems Symptom Probable Cause Resolution Dispense volumes are incorrect. The dispense pins are leaking. Check the pins, seals and tubing for leaks. The dispense pressure is low. Check the seals and tubing for leaks. Check for air leaks. Reseat the tubing in the dispense valve. Aspiration is not complete. The dispense pins are clogged. Remove the wash head assembly and clean it using the manifold head cleaning wire. Then run a purge. Service is required if problem persists.
Operational Problems Symptom Probable Cause Resolution Aspiration is not complete. (continued) Hydrophobic filter may be obstructed. Replace filter. High Waste Level is displayed. The Waste Container is full. Empty the Waste Container (see page 70). The waste level sensor cable is not plugged in. Connect the waste level sensor cable (see page 17). The waste level sensor connector is defective. Service is required. The waste level sensor detector is reversed. Service is required.
Service Procedures Chapter 8 Service Service Procedures The only user service procedures that can be carried out for the Opsys MW™ are listed below: • Removal and replacement of the wash head assembly • Cleaning of the wash head assembly To remove the wash head assembly: 1. Loosen the two thumbscrews at the front of the Arm/Head assembly. 2. Grasp the wash head assembly and lift it up from the retaining pins on the Arm. 3. Lift the wash head and tubing clear of the instrument.
Spare Parts Spare Parts Accessories 13001580 13001610 13001630 352104000 352101800 91000051 91000070 23501800 029006501 029027400 200074300 394000400 32000170 43000200 43000390 43000491 43000540 816400500 816400600 816400700 62503160065 43000540 Plate Holder Assembly 8-Way Head Assembly 12-Way Head Assembly Cleaning Wire, 0.040” Cleaning Wire, 0.
Returning a Washer for Service Returning a Washer for Service If the instrument must be returned for service, it must be cleaned and decontaminated if it has been in contact with potentially infectious body fluids (including human blood), pathological samples, or toxic or radioactive materials. Note: Refer to page 69 for cleaning and decontamination instructions. To return a Washer for service: 1. Contact the nearest DYNEX Technologies technical service facility for return authorization. 2.
Returning a Washer for Service EQUIPMENT IN TRANSIT IMPORTANT: Please include a copy of this form with each instrument. If your instrument contains a hard drive, please retain back-up copies of any stored files. Failure to do so may result in the loss of those files. Return Authorization Number: ___________ Contact Technical Service, DYNEX Technologies phone: (800) 288-2354 or (703)631-7800 ext.
Contact DYNEX Technologies Contact DYNEX Technologies DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Tel: 703-631-7800 Toll Free: 800-288-2354 Fax: 703-803-1441 www.dynextechnologies.
Index Index as required maintenance, 68 ASPIRATE, 39 aspiration height, 4 entering, 32 bottom wash specifying, 34 bottom wash height, 4 CAL.
Index variable strips, 41 warning labels, 11 Wash Container, 5, 16 cleaning, 64 installing, 20 refilling, 64 wash fluid, 5 wash head, 3, 15 cleaning, 81 installing, 19 positions, 4 removal, 81 replacement, 81 types, 3 wash pins, 3 wash pins, 3 Opsys MW™ User’s Guide wash protocol copying, 61 deleting, 59 inserting, 60 modifying, 56 operations, 39, 45 wash protocols programming, 2 washing a plate, 63, 65 waste, 5 Waste Container, 16 cleaning, 63 emptying, 63 waste tubing, 73 weekly maintenance, 67 well top
