User Manual

SECTION 8 – Quantitation
The quantitation measurement mode enables sample concentrations to be calculated using a standard
curve. In this mode a number of standard solutions covering a range of known concentrations are
measured at a set wavelength. The absorbance or % transmittance of these solutions is plotted to create
a standard curve with replicate standards measurements, if required. Once the standard curve has been
created a sample of unknown concentration can be measured and the concentration calculated using
the standard curve.
8.1 MODE SPECIFIC PARAMETERS
The quantitation operating menu enables measurement
parameters to be changed. The utility toolbar on the left
hand side of the screen enables access to printing, print
setup options, results, methods and autologging options.
For more details on the different functions of the utility
toolbar refer to section 17.
The quantitation settings menu enables the absorbance or % transmittance operating mode, wavelength,
units, resolution, number of replicate measurements (manual or automatic) and number of calibration
standards to be set. The quantitation table menu enables the quantitation standard data to be viewed,
edited and allows a new curve to be created. The standard curve menu enables a standard curve to be
created, viewed and edited.
Fig 8.1.1 - Operating Menu – Post Calibration
8.2 METHOD SETUP
In this measurement mode the method setup parameters
are accessed through the quantitation settings menu. To
open the quantitation settings menu press the key adjacent
to the quantitation settings icon in the operating menu.
Operating Menu
Print/print settings
Results selection menu
Method selection menu
Autolog menu
Calibrate
to zero
Measure
sample
Quantitation settings
Quantitation table
Standard curve
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