User Manual

SECTION 9 – Kinetics

The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be

measured over a period of time; for example enzyme analysis of horseradish peroxidase. The absorbance

or % transmittance is measured at regular time intervals at a set wavelength over a period of time.

The results are plotted on a graph to show the change in absorbance or % transmittance over time.

Following sample measurement statistical analysis of all or part of the experiment can be performed.

9.1 MODE SPECIFIC PARAMETERS

The kinetics operating menu enables measurement

parameters to be changed. The utility toolbar on the left

hand side of the screen enables access to printing, print

setup options, results, methods and autologging options.

For more details on the different functions of the utility

toolbar refer to section 17.

The settings icon enables the wavelength, units, resolution, graph y-axis, absorbance or % transmittance

operating mode, standard, factor, measurement time, lag time and start on level to be set. Following

the completion of a set of kinetics measurements the statistics for all or part of the kinetics run can be

analysed. The kinetics moving line function enables the initial and ﬁnal points of the curve to be selected,

so that analysis of the area between these two points can be performed.

Fig 9.1.1 - Expanded Operating Menu – Post Measurement

9.2 METHOD SET UP

In this measurement mode all the method set up parameters

are accessed through the settings menu. To open the

settings menu press the key adjacent to the settings icon

in the operating menu.

Operating Menu

Print/print settings

Results selection menu

Methods selection menu

Autolog menu

Calibrated

to zero

Start kinetics

measurements

Settings

Kinetics moving line

Statistics