User Manual

14.6 260 / 280

The 260/280 operating menu enables the purity and

concentration of DNA to be estimated according to the

following equations:-

Purity Ratio = Abs@260nm / Abs@280nm

Conc (μg/ml) = (Abs@260nm x 62.9) - (Abs@280nm x 36.0)

The 260/280 settings menu allows the user to adjust

measurement speciﬁc parameters such as the dilution factor

and resolution but the concentration units are limited to

µg/ml and mg/ml only. Other method settings, such as the

inclusion of a reference wavelength and the concentration

equation and factors can be altered if required, but the

260/280 method settings are locked and any changes

will be lost after exiting the mode. See Section 13 for full

instructions on altering the 260/280 method options and

settings.

14.7 260 / 230

The 260/230 operating menu enables the purity and

concentration of DNA to be estimated according to the

following equations:-

Purity Ratio = Abs@260nm / Abs@230nm

Conc (μg/ml) = (Abs@260nm x 49.1) - (Abs@230nm x 3.48)

The 260/230 settings menu allows the user to adjust

measurement speciﬁc parameters such as the dilution factor

and resolution but the concentration units are limited to

µg/ml and mg/ml only. Other method settings, such as the

inclusion of a reference wavelength and the concentration

equation and factors can be altered if required, but the

260/280 method settings are locked and any changes

will be lost after exiting the mode. See Section 13 for full

instructions on altering the 260/230 method options and

settings.

14.8 VARIABLE RATIO

The Variable Ratio operating menu enables the purity and

concentration of DNA to be estimated according to the

following equations:-

Purity Ratio = Abs@Xnm / Abs@Ynm

Conc (μg/ml) = (Abs@Xnm x f1) - (Abs@Ynm x f2)

Where X, Y, f1 and f2 are deﬁned by the user.

Operating Menu