Leica StereoFluorescence Systems User Manual
Contents Page Leica MZ10 F fluorescence stereomicroscope Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Fluorescence applications . . . . . . . . . . . .
Description Safety concept Fluorescence technique Before you set up the instrument and before you fit the fluorescence illuminator, read: • this user manual, observing the notes relating to safety Some substances fluoresce when irradiated with short-wave light. This property is utilized in the fluorescence technique, in which certain structures and features which do not fluorescence can be tagged with a fluorescing dye.
Servicing and repairs Supply unit – Repairs may only be carried out by Leica-trained service technicians or by technical personnel authorized to do so by the person in charge of the instrument • Pull the power plug of the supply unit out from the power soc- – Only original Leica spare parts may be used – before opening the lamp housing – Unplug the power cable before opening the supply unit. Touching live circuits can cause injury.
Applications Natural science Application Fluorescence filter set Anatomy • Monitoring of capillary flow • GFP 1, GFP 2 Biology • Gene expression in chicken embryos, fruit flies, threadworms and zebra fish • Fish otoliths marked with alizarine red • Genetic expression through viruses and bacteria which infect plants and animals • GFP 1, GFP 2 • Green • GFP 2 Biomedicine • Humatic seals on pacemakers • Blue, green Genetics • Cellular detection and protein expression • S
Industry Application Fluorescence filter set Electronics • Solder paste on SMDs • Epoxy resin on SMD plates • Luminescent coating of TV monitor tubes • Quality of polymer castings for embedding integrated circuits • GFP 1, GFP 2 • GFP 1, GFP 2 • GFP 1, GFP 2 • Violet Semiconductors • Positive photo resists (OCG HPR 504) • Negative photo resists • Detection of foreign particles on masks, wafers and • subassemblies • GFP 1, green • Blue • UV, GFP 1, GFP 2 • Violet Petrochemic
Overview Leica MZ10 F: Its components and controls 1. Choice of stand and of focusing drive (manual or motor-driven) 2. Microscope carrier for Leica MZ10 F 3a. Leica MZ10 F optics carrier with third beam path 3b. Integrated FLUOIII filter system 3e 4a 3c 8 3b 3d 3c. Adapter for light source 3d. Rapid filter changer for any of four sets of filters 3e. Slots for light stop and for filter slide with individually-selectable filter 4a.
Filter sets for Leica MZ10 F Designation Excitation filter Barrier filter GFP fluorescence GFP1 425/60 nm 480 nm GFP Plus fluorescence GFP2 480/40 nm 510 nm GFP plant fluorescence GFP3 470/40 nm 525/50 UV fluorescence UV 360/40 nm 420 nm Violet fluorescence V 425/40 nm 475 nm Blue fluorescence B 470/40 nm 515 nm Green fluorescence G 546/10 nm 590 nm Filter set GFP for LEICA MZ10 F 100% Excitation Filter (425/60) Barrier Filter (480) Transmission Filt
Leica MZ10 F 5a. Lamp housing 106Z Assembly 5b. Stray-light protection 1. Incident- or transmitted-light base 6. Binocular tube 2a. Manual focusing drive, or 7. UV protection screen with arm 2b. Motorized focusing drive 8. 4 sets of filters 3. Microscope carrier for Leica MZ10 F 4. Leica MZ10 F optics carrier with FLUOIII™ filter system 9. Light stop 10. Filter slide for an individually-selectable filter 11. Double-iris diaphragm (optional) 12.
Leica MZ10 F UV protection screen ➜ microscope carrier Focusing drive ➜ base of stand ▶ Connect manually-operated or motor-driven focusing drive, complete with column, to base, as described in user manual M2-105-0EN. Before using the motor focus, you must read the safety directions in the accompanying user manual M2-267104. Microscope carrier ➜ focusing drive ▶ Fit microscope carrier of Leica MZ10 F to focusing drive in accordance with user manual M2-105-0EN.
Assembly ▶ Slacken the Phillips screws Lamp housing ▶ Carefully extract the socket, complete with burner mount and connecting cable The Leica 106Z lamp housing is equipped. For information about the supply units for the 106Z lamp housing, see page 14. Model 105Z lamp housings, if available, can also be used.
▶ Using the focusing knob, displace the collector: • During this movement, the collector must not touch the flexible power cable. If necessary, bend the cable away Removing the collector and the heat-protection filter The collector and the heat-protection filter can be removed for cleaning or if defective.
Assembly 3 4 5 Supply unit for lamp housing 106Z with 50W mercury-vapour burner 1. On/off switch 2. Running-time meter 3. Selector for L1/L2 burners 4. Fuse 5. Selector for 50Hz/60Hz 6. Connector for lamp 7. Power cable 2 1 6 7 Input type 220V/240V ±10% 220V ~50/60Hz Hg50W L1/L2 Osram no. 0-958 Max. power 320VA The supply unit is intended for power supplies above 220V. To run it on lower voltages a step-down transformer is required (e.g. 230V to 110V).
Adjusting the mercury vapour lamp This adjustment is important for obtaining a uniform light spot and good-quality fluorescence. While adjusting the mirror image, do not project light on to the electrodes for long periods (risk of explosion through overheating) The two electrodes are imaged as an extension of the plane of symmetry of the discharge arc, but are difficult to see.
Leica MZ10 F Dummy filter carrier FLUOIII filter system In the dummy filter carrier there are two openings for the observation beam paths instead of the barrier filters. The third path has been closed. Use the dummy filter carrier: – if you are using less than four sets of filters The filter system consists of a rapid filter changer for the barrier- and excitation filters and two slots for a light stop and for a filter slide respectively.
Use ▶ Illuminate a flat test object with transmitted light or with oblique incident light. MZ10 F: Bringing the specimen into focus and observing it ▶ Carry out the dioptric correction as directed in the user manual for the stereomicroscope. Observe the safety directions on page 4 and the recommendations on page 5 regarding the use of the mercury-vapour lamp. Changing filters Observing fluorescence Make sure that the UV protection screen is positioned correctly (page 11).
Overview Outfit with stereo-fluorescence module The components 1 Choice of stand and of focusing drive (manual or motor-driven) 5a Leica 106Z lamp housing for 50W or 100W mercury-vapour burners 5b Stray-light protection 3 Leica MS5, MZ6, MZ7.
Filter sets for stereo-fluorescence module Filter sets Excitation filters Dichromatic Barrier beam splitters filters GFP fluorescence 425/60nm 470nm GG475 GFP-Plus fluorescence 480/40nm 505nm LP 510nm LP GFP-Plant 470/40nm 495nm 525/50nm UV fluorescence 360/40nm 400nm GG420 Violet fluorescence 425/40nm 460nm GG475 Blue fluorescence 470/40nm 505nm OG515 Green fluorescence 546/10nm 565nm OG590 19
Assembly Stereo-fluorescence module 7 Binocular tube 8 Clamp 1 Incident- or transmitted-light base 9 Arm 2a Manual focusing drive, or 10 UV-light excluder 2b Motorized focusing drive 11 Filter carrier 3 Microscope carrier 12 Filterholder for excitation filter 4 Choice of optics carrier 13 Light stop 5 Fluorescence module 14 Filter carrier with an individually-selectable filter 6a 106Z lamp housing 15 Double-iris diaphragm (optional) 6b Stray-light protecti
Stereo-fluorescence module Focusing drive ➜ base of stand ▶ Connect manually-operated or motor-driven focusing drive, complete with column, to base, as described in user manual M2-105-0EN. Before using the motor focus, you must read the safety directions in the accompanying user manual M2-267-104. 3 Important: With the illuminator on, insert the filterholder with diaphragm into slide-in unit 3 to avoid being suddenly exposed to excessively bright light (page 22). ▶ Remove the binocular tube.
Assembly Filterholder ➜ fluorescence module Stereo-fluorescence module UV-light excluder ➜ side-faced column Always position the UV-light excluder so that the user never looks directly at the light spot (page 5). The dichromatic beam splitter and the barrier filter are built into the fluorescence module in accordance with the configuration (see table, page 19). The excitation filter is supplied in a separate filterholder. A filterholder with diaphragm is also supplied.
Use Observation Stereo-fluorescence module Focusing and observing Observe the safety regulations on page 4 and note the recommendations on page 5 for using the mercuryvapour burner. Position the UV-light excluder correctly (see page 22): ▶ First use the lowest magnification to observe the object • You will gain a better overall view and can more easily find interesting features ▶ Refocus slightly if necessary ▶ Observe details at a higher magnification.
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