BioOptix™ 10 Installation and Operation Guide #69-5203-150 Copyright © 2004 Teledyne Isco, Inc. All rights reserved.
Foreword This instruction manual is designed to help you gain a thorough understanding of the operation of the equipment. Teledyne Isco recommends that you read this manual completely before placing the equipment in service. Although Teledyne Isco designs reliability into all equipment, there is always the possibility of a malfunction. This manual may help in diagnosing and repairing the malfunction. If the problem persists, call or e-mail the Teledyne Isco Technical Service Department for assistance.
BioOptix™ 10 Safety BioOptix™ 10 Safety General Warnings Before installing, operating, or maintaining this equipment, it is imperative that all hazards and preventive measures are fully understood. While specific hazards may vary according to location and application, take heed in the following general warnings: WARNING Liquids associated with this instrument may be classified as carcinogenic, biohazardous, flammable, or radioactive.
BioOptix™ 10 Safety AVERTISSEMENT Ce système peut utiliser des dissolvants organiques inflammables. Pour réduire le péril qui peut être causé par l'accumulation des vapeurs explosives, Teledyne Isco recommande que vous installez ce système dans un environnement bien-aéré qui est conçu pour les matières hasardeuses. Cet environnement devrait être construit selon les règlements fédéraux, provinciaux, et locaux.
BioOptix™ 10 Safety Hazard Symbols The equipment and this manual use symbols used to warn of hazards. The symbols are explained below. Hazard Symbols Warnings and Cautions The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument’s technical reference manual. The lightning flash and arrowhead within the triangle is a warning sign alerting you of “dangerous voltage” inside the product.
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BioOptix™ 10 Table of Contents Section 1 Introduction 1.1 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.3 System Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.3.1 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
BioOptix™ 10 Table of Contents 3.8 Post-experiment Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.8.1 Pump Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.8.2 Saving Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.8.3 AutoReports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
BioOptix™ 10 Table of Contents List of Figures 1-1 1-2 1-3 1-4 2-1 2-2 2-3 2-4 2-5 2-6 2-7 2-8 2-9 BioOptix 10 front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 BioOptix 10 left-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4 BioOptix 10 right-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5 BioOptix 10 rear panel controls and connections . . . . . . . .
BioOptix™ 10 Table of Contents List of Tables 1-1 1-2 1-3 1-4 1-5 2-1 2-2 3-1 4-1 x BioOptix 10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 BioOptix 10 Left-side Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4 BioOptix 10 Right-side Connections . . . . . . . . . . . . . . . . . . . . . . .
BioOptix™ 10 Section 1 Introduction 1.1 Product Overview The BioOptix™ 10 system performs automated protein purification on as many as ten liquid samples simultaneously. The system has ten independent pumps that keep flow rates properly regulated in each channel, and ten independent detectors so that you can monitor the separation in every channel.
BioOptix™ 10 Section 1 Introduction 1.2 System Specifications Table 1-1 lists the BioOptix 10 specifications. Table 1-1 BioOptix 10 Specifications Dimensions a Height: 69 cm Width: 72 cm Depth: 61 cm Weight a,b Power Requirement 210 kg a,c The power switch is the disconnect device. Line Frequency Operating Temperature Humidity 100 VAC ±10%, 2.0 Amps 117 VAC ±10%, 2.0 Amps 234 VAC ±10%, 1.
BioOptix™ 10 Section 1 Introduction 1.3 System Controls and Connectors 1.3.1 BioOptix 10 Front Panel Controls 1 2 3 4 5 Figure 1-1 BioOptix 10 front panel Table 1-2 BioOptix 10 Front Panel Controls Item Name Description 1 Manual Sample Inject Port Injection port for syringe-injected liquid samples. 2 Mode Knob Configures fluid path for purging, manual sample injection, or purification operation. 3 Column Inlet Port Allows connection of tubing to column inlet.
BioOptix™ 10 Section 1 Introduction 1.3.2 BioOptix 10 Left-side Controls and Connections 1 2 4 3 5 6 7 Figure 1-2 BioOptix 10 left-side connections Table 1-3 BioOptix 10 Left-side Connections Item Name 1-4 Description 1 Buffer A Inlet Ports Ports to accept Buffer A solution for each channel. 2 Buffer B Inlet Ports Ports to accept Buffer B solution for each channel. 3 Sample In Ports Ports to accept sample for each channel.
BioOptix™ 10 Section 1 Introduction 1.3.3 BioOptix 10 Right-side Connections 1 Figure 1-3 BioOptix 10 right-side connections Table 1-4 BioOptix 10 Right-side Connections Item Name 1 Purified Sample Outlet Description Channels 1 through 10 which connect to the Foxy 200 Fraction Collector.
BioOptix™ 10 Section 1 Introduction 1.3.4 BioOptix 10 Rear Panel Controls and Connections 1 2 3 4 Figure 1-4 BioOptix 10 rear panel controls and connections Table 1-5 BioOptix 10 Rear Panel Controls and Connections Item Name Description 1 Cooling Fan Ventilates the BioOptix 10 case and cools internal components. 2 External Control Connector Connects to the Foxy 200 Fraction Collector and computer. 3 Fuses Rear Panel Accessible Fuses.
BioOptix™ 10 Section 2 Installation This section provides instructions for installing the system and preparing it for operation. The BioOptix 10 system consists of a BioOptix 10 unit, a Foxy 200 Fraction Collector, and a Windows PC running BioOptix 10 software. WARNING The system is heavy. Use a two-person lift to prevent injury. 2.1 Unpacking the BioOptix 10 System Carefully unpack the shipment and inspect the contents to verify receipt of all components against the packing list.
BioOptix™ 10 Section 2 Installation To keep tubing length at a minimum, buffer, sample, and waste collection containers should be placed to the left of the unit. The containers may also be kept behind the unit as long as the containers are accessible. The Foxy 200 should be installed to the right of the BioOptix 10. The fraction collector requires about 1800 square centimeters (275 in 2 ) of bench space. Ensure that the full motion of the fraction collector arm is not obstructed.
BioOptix™ 10 Section 2 Installation Pump Module Column Module Figure 2-2 Accessing pump and column module internal connections To complete the connections: 1. Release the two sets of tubes from the retaining clips (Figure 2-3). 2. Remove the four screws securing the back metal plate on the column module (Figure 2-3). Then, lift and remove the plate.
BioOptix™ 10 Section 2 Installation 3. While facing the back of the column module, locate the two liquid tubing bundles secured to the lower-left inside case panel. Route these two bundles through the slots at the edge of the panel (Figure 2-4). Route tubing through slots To Foxy 200 Figure 2-4 Routing the Sample Out lines (column module) 4. Connect the beige 25-pin connector from the pump module to the column module connector labeled Pump Controller. Refer to Figure 2-5.
BioOptix™ 10 Section 2 Installation 5. Connect the orange connector labeled BD3 P110 to P110 on the Power Drive Circuit Board (Figure 2-6). From Pump Module Figure 2-6 P110 Connection on BD3 6. Connect the plug labeled BD1 J106 to J106 on the Controller Circuit Board (Figure 2-7).
BioOptix™ 10 Section 2 Installation 7. Plug the black power cord from the pump module into the receptacle at the lower part of the column module (Figure 2-8). From Pump Module Figure 2-8 AC power connection from pump module 8. Before completing the remaining cable connections, replace the back metal plate on the column module. The openings in the plate will fit around the connections made in steps 3 through 7. Secure the plate with the four screws (Figure 2-3).
BioOptix™ 10 Section 2 Installation 10. Connect the tubing from the column module to the pump module. Refer to Figure 2-10. a. Remove the two thumbscrews securing the splash guard to the pump module. Set aside the splash guard. Note The tubing numbers from the column module match the numbers above the compression fittings on the pump module. (The tubing is visible in Figure 2-9; the compression fittings are visible in Figure 2-10.) b.
BioOptix™ 10 Section 2 Installation Figure 2-11 Aligning the fasteners Figure 2-12 Column clamps inserted 2-8
BioOptix™ 10 Section 2 Installation 12. Ensure the Power Switch is in the Off (“0”) position. Then, connect the AC power cord to the back of the Pump Module. Connect the other end of the power cord to an outlet meeting the electrical requirements listed on the serial number label. 13. Locate the Serial “Y” cable, P/N 60-1020-241 in the Accessories Kit. Connect the 25-pin connector labeled First Instrument Optix to the External Control port on the back of the Pump Module.
BioOptix™ 10 Section 2 Installation 5. Connect the AC power cord to the back of the Foxy 200. Connect the other end of the power cord to an outlet meeting the electrical requirements listed on the serial number label. 6. Connect the “Y” Serial Cable end labeled Second Instrument Foxy to the RS-232-C port on the back of the Foxy 200. 7. Place the Foxy 200 to the right of the BioOptix 10 (Figure 2-1).
BioOptix™ 10 Section 2 Installation Common Buffer Inlet Mounting Block Manifold Figure 2-14 Buffer assembly routes a common solution to all ten channels Figure 2-15 Buffer assemblies installed To connect the Buffer Assembly: a. Remove the two screws located in the center of the Buffer A inlet ports. (The two exposed holes are used to mount the Buffer Assembly.) b. Attach the mounting block to the two holes using the hex head screws. c.
BioOptix™ 10 Section 2 Installation Buffer B – To form gradient buffers during purification, connect Buffer B solution(s) to these ten inlet ports. Perform the steps described in Buffer A above to complete these fluid connections. Sample In – The ferrules on these inlet ports accept the 0.063" OD tubing. The accessory kit contains Teflon tubing of this size in five different colors.
Blue Yellow Orange Green Clear Blue Yellow Orange Green Clear BioOptix™ 10 Section 2 Installation 1 2 3 4 5 6 7 8 9 10 10 1 Figure 2-16 Foxy 200 tubing connections Refer to Figure 2-16 and complete the tubing connections between the BioOptix 10 and the Foxy 200. Thread and finger-tighten the fittings into the ten-channel drop former assembly. After connecting the tubing, peel the adhesive backing from each retainer.
BioOptix™ 10 Section 2 Installation 2.2.6 Windows PC Connection The Windows® personal computer (PC) must meet the following minimum requirements: Table 2-2 Windows® PC Minimum Requirements Processor: Pentium, 133 MHz RAM 32 MB Hard Drive 20 MB available space Operating System Windows® 95, 98, 2000, NT, or XP Communication Port One available RS-232 port CD-ROM 16× Monitor VGA or better (640×480 or higher resolution) The PC should be set up according the manufacturer’s instructions.
BioOptix™ 10 Section 2 Installation ❍ Rack Change Bell – When this option is enabled, the system provides an audible signal while it is waiting for the operator to change the tube rack. ❍ Com Port – select the Com Port number to which the “Y” Serial cable is connected to the PC. Refer to the documentation provided with your PC. ❍ Fraction Size – type or select the desired fraction size to be collected by the Foxy 200. ❍ Stroke Volume – type or select the desired stroke volume of the pump.
BioOptix™ 10 Section 2 Installation 6. Ensure that each channel on the BioOptix 10 has 0.125" OD bypass tubing between the Column Inlet and Outlet ports (Figure 2-18). Column Inlet Column Outlet Figure 2-18 Column bypass tubing 7. For each channel, set the mode control knobs to Purge (Figure 2-19). Figure 2-19 Mode control knob 8. From the BioOptix 10 software menu, select Tools>Manual Control. The BioOptix 10 Manual Hardware Control window is displayed (Figure 2-20).
BioOptix™ 10 Section 2 Installation Figure 2-20 BioOptix 10 Manual Hardware Control window 9. In the Columns section of this window, check each channel number that should be primed. Configure the remaining settings to match those shown in Figure 2-20. 10. Click the Start Pump button. Observe the following for each enabled channel: ❍ Buffer B solution movement should be visible at the inlet ports on the left side of the BioOptix 10.
BioOptix™ 10 Section 2 Installation 2-18
BioOptix™ 10 Section 3 Operation This section provides an introduction to preparing the system and creating files necessary for a purification run. The instructions assume that the system has been set up according to Section 2. 3.1 Sample Preparation The Sample In lines for each channel should be placed into a vessel holding the liquid sample mixture. Sample In lines can be placed into a common sample vessel, or in multiple vessels when unique samples are to be introduced on each channel. 3.
BioOptix™ 10 Section 3 Operation 3.3 BioOptix 10 Software Note Refer to On-line Help for specific BioOptix 10 software operating instructions. 3.3.1 Overview The operation of the BioOptix 10 system is directed by three files: • Gradient – these files define how a gradient is to be formed from Buffer A and B solutions. • Method – these files contain the settings that direct the operation of each channel of the system. A method file will reference the settings in a gradient file.
BioOptix™ 10 Section 3 Operation Note The percent B value is the amount of Buffer B solution mixed with Buffer A. A value of zero percent B (0 %B) pumps only Buffer A. Conversely, a value of 100 %B pumps only Buffer B. All other rows in the table define the %B that will be reached after pumping the listed volume. The system forms a linear gradient between inflection points. This can be observed in Figure 3-1.
BioOptix™ 10 Section 3 Operation The default method file contains five blocks that are appropriate for use with affinity column purification: • Equilibrate – conditions the column. • Load – loads the sample onto the column. • Wash – washes compounds from the column that do not bind to the column media. • Elute – pumps a buffer solution to release the purified compounds from the column.
BioOptix™ 10 Section 3 Operation Pausing at the end of a block allows for buffer changes or other mechanical adjustments before proceeding with the next block. • Wavelength – Type or select the desired wavelength from 190 to 360 nm to be used for UV detection. Note that all blocks use the same wavelength. Note The Equilibrate tab is a “Fluid” block type. See Appendix A. 3.5.2 Load Settings This block loads the sample onto the column.
BioOptix™ 10 Section 3 Operation 3.5.3 Wash Settings This block flushes compounds that did not bind to the affinity media. The Wash tab is a fluid block type, therefore the settings displayed on this tab are the same as the Equilibrate tab. Refer to section 3.5.1 for a discussion on the settings for this tab. Figure 3-4 Wash tab settings 3.5.4 Elute Settings This block pumps the buffer solutions through the column. The mixture and quantity of buffer solution is defined by the gradient file.
BioOptix™ 10 Section 3 Operation • Collect Effluent – Select the desired action for effluent fluid as it comes off the column. If Yes is selected, enter the desired fraction size. Note The Elute tab is a “Gradient” block type. See Appendix A. 3.5.5 Flush Settings This block pumps fluid to flush remaining sample from the column and system tubing. This step can prepare the column for the next purification run, storage, or disposal.
BioOptix™ 10 Section 3 Operation 3.6.1 Setting up the Experiment Before proceeding, determine which BioOptix 10 channel positions will be used during the purification run. Channels that will be in use require that the corresponding rows in the table be completed. Note that to use these positions they should have samples prepared (section 3.1) and columns installed (3.2). Table rows for unused channels must remain empty. To set up the experiment: 1.
BioOptix™ 10 Section 3 Operation 3.6.3 Saving the Experiment File 3.7 Running the Experiment Save the experiment by selecting the File>Save menu command. Use a descriptive file name so that it can be located for later use. With the experiment file open, some BioOptix 10 operation buttons along the bottom of the window are active (Figure 3-8). Buttons will be active or inactive according to the current state of the system.
BioOptix™ 10 Section 3 Operation Figure 3-9 Pause at end of block If the block is set to collect effluent (Figure 3-4), the fraction collector will deposit the fluid in the collection tubes. If a peak is detected, the fraction collector automatically advances to the next row to isolate the fluid. The fraction collector will also automatically advance to the next row of tubes whenever the specified fraction size has been collected.
BioOptix™ 10 Section 3 Operation The Fast Forward button is active after clicking the Stop button. The Fast Forward button allows you to skip to the next block in the experiment or abort the run. 3.8 Post-experiment Operation 3.8.1 Pump Flush At the completion of the experiment, the BioOptix 10 provides the option to flush the pumps (Figure 3-10). The pump flush will pass a wash fluid through the pumps to prevent salts from crystallizing in the system.
BioOptix™ 10 Section 3 Operation 3. Select a wash fluid. The wash fluid must be compatible with the buffer system and of sufficient strength to dissolve salts. If this solution is different than the buffers, move the Buffer In lines to the wash fluid container. 4. For all selected channel positions, turn the Mode knob to the purge pump position. 5. Click the Start button to begin the flush routine. The BioOptix 10 flushes the pumps. 6.
BioOptix™ 10 Section 3 Operation 3.8.3 AutoReports After the run the BioOptix 10 can automatically print the purification run results or save the file in a format listed in section 3.8.2. To automatically receive the hard copies or save the files, select the Tools>AutoReport menu command and modify the settings.
BioOptix™ 10 Section 3 Operation 3-14
BioOptix™ 10 Section 4 System Maintenance 4.1 Periodic Maintenance The BioOptix 10 system’s internal components have been selected for long life and low maintenance. To maintain the system, the only required periodic maintenance is to flush the pumps daily. The pump flush passes a wash fluid through the pumps to prevent salt buffers from crystallizing in the system. CAUTION Salts will cause premature wear to the internal components and eventually equipment failure.
BioOptix™ 10 Section 4 System Maintenance The BioOptix 10 system pumps the wash fluid, flushing the Buffer In lines, gradient formation components, pump, and purge lines. (See Figure B-6 in Appendix B.) 4.2 Exterior Cleaning For general cleaning of the enclosure, use distilled water with a mild detergent. Use isopropyl alcohol for tougher stains. Avoid rubbing vigorously or using aggressive solvents like acetone on printed areas. CAUTION Do not immerse the instrument in a water bath.
BioOptix™ 10 Section 4 System Maintenance 2 4 3 1 Figure 4-2 Access the pump assembly Note Refer to Figure 4-3 for the following steps. 9. Remove the bottom retainer plate. 10. On the top of the pump assembly, remove the two 10-32 × 3/8" pan head screws and lock washers. 11. Lift the piston plug and top O-ring from the glass cylinder. 12. Remove the glass cylinder and bottom O-ring. This exposes the push rod and seal. 13. The push rod shaft has two slots for a wrench.
BioOptix™ 10 Section 4 System Maintenance 10-32 × 3/8" Pan Head Screw #10 Interior Lock Washer #10 Flat Washer Piston Plug Top O-Ring (P/N 202-5001-09) Glass Cylinder Inductor #8-32 × 1-1/4" Pan Head Screw Top Mounting Tab Seal (P/N 202-9090-32) Push Rod Bottom O-Ring (P/N 202-5001-09) Wrench Slots Bottom Mounting Tab .375 OD Flat Washer .5 OD Rubber Washer .
BioOptix™ 10 Section 4 System Maintenance 17. Hold the push rod upside-down and insert it into the opening on the seal. Apply downward force until the seal snaps onto the push rod. 18. Refer to Figure 4-3 and reassemble the pump channel using new O-rings at the top and bottom of the glass cylinder. 19. Repeat steps 10 through 18 for the remaining channels. Note Channel six is located below the inductor.
BioOptix™ 10 Section 4 System Maintenance CAUTION Hot surfaces may cause burns. The UV lamp and nearby surfaces are hot. To remove the lamp: 1. Remove the two screws that secure the waste port mounting plate (Figure 4-5). The optical assembly is located behind this plate. Figure 4-5 Waste port mounting plate Lamp Mounting Screws Twist Lock Clamp Figure 4-6 Deuterium lamp location 2. Disconnect the wiring connector. 3. Release the twist lock cable clamp. 4. Remove the three lamp mounting screws. 5.
BioOptix™ 10 Section 4 System Maintenance Replacing the lamp To install the new lamp: CAUTION Contaminants on the glass bulb will shorten the life span. Oil from your skin is one such contaminant. Wear clean cotton gloves when handling the deuterium lamp. 1. Carefully insert the replacement bulb (part number 69-5203-014) into the optical assembly and secure it with the lamp mounting hardware. 2. Route and secure the wires through the twist lock clamp. 3. Reconnect the wiring connector. 4.
BioOptix™ 10 Section 4 System Maintenance 4.5.2 Leaks If leaks occur or if solid material is observed around a fitting, determine the source of the leak and tighten the fitting. Note When you tighten a fitting, make sure that you do not overtighten it. Overtightening a fitting may lead to permanent damage and require replacement of the fitting. 4.5.3 Electrical 1. If your instrument stops working, check the line cord connection. 2.
BioOptix™ 10 Section 4 System Maintenance WARNING Risk of damage to eyesight and skin. Wear ultraviolet (UV) light eye protection and protect skin from exposure if the deuterium lamp is operating. CAUTION Hot surfaces may cause burns. The UV lamp and nearby surfaces are hot. To reset the circuit breaker, separate the pump and column modules (see section 2). Locate the circuit breaker illustrated in Figure 4-7 and press the button.
BioOptix™ 10 Section 4 System Maintenance 4.6 Service Department If you have a problem with the instrument or need parts information, contact Isco’s Service Department. If you write, please include all pertinent information that may be helpful in solving your problem. Address your letter to: Teledyne Isco, Inc. Service Department P.O. Box 82531 Lincoln, NE 68505 Before deciding to return the unit for factory repair, we suggest that you call the Service Department.
BioOptix™ 10 Appendix A Method Templates When creating a new method, the BioOptix 10 software configures the blocks of the method file according to a template file. This template file can be modified, allowing it to be customized for many applications. A method can have as few as one block or as many as ten. A block can be created to pump an isocratic mix of buffers, pump a gradient mix of buffers, or load a sample. These differing types of blocks can occur in any desired order.
BioOptix™ 10 Appendix A Method Templates 2. From Notepad’s menu select File>Open. 3. Change the Files of type pull-down list to All files. 4. Use the Open dialog box to locate the Standard.tpl file. Default installations of BioOptix 10 software place this in the Program Files folder on the hard drive. Typically the path is C:\Program Files\BioOptix 10\Standard.tpl. Notepad will then display the contents of the template file. The Standard.
BioOptix™ 10 Appendix A Method Templates 5. Check the box labeled Always use this program to open these files. 6. Click OK. After doing so, double-clicking on any file with the .tpl filename extension will open it in the selected text editor. A.2 Modifying the Template Once open in a text editor, the template file can be modified. Refer to Figure A-2 and the discussion following for instructions on modifying the template.
BioOptix™ 10 Appendix A Method Templates Additional rules should be observed and are described in the following sections. A.2.2 [Blocks] The count in this section should equal the total number of blocks in the template. When generating a method, the number of tabs created will equal the count value. The acceptable value range is 1 to 10. A.2.3 [Blockn] This section specifies what information appears on a tab.
BioOptix™ 10 Appendix B Fluid Path Diagrams This appendix contains simplified diagrams of the plumbing and valves internal to the BioOptix 10 system. Various operating states are depicted for a single channel. A complete plumbing diagram can be found in Figure B-7. An understanding of fluids paths can be helpful when designing custom template files (Appendix A), manually controlling the system, or troubleshooting.
BioOptix™ 10 Appendix B Fluid Path Diagrams While the system is running a fluid-type block: • The Buffer valve switches between buffers A and B to produce the correct mix. • The Sample valve allows only buffer solution to pass. • If the channel is active, the Channel valve allows the fluid to be pumped. If the channel is inactive, the valve prevents fluid from being pumped (Figure B-2). • The Mode valve is in the Operate position.
BioOptix™ 10 Appendix B Fluid Path Diagrams Manual injections do not operate the pump. The system pauses for the operator to turn the Mode valve to the Manual Inject position. The liquid sample is then forced through the injection port on top of the BioOptix 10 system. The liquid sample is introduced directly onto the column (Figure B-4). Manual Sample Injection Mode Column Effluent To Foxy Figure B-4 Fluid path for Load-type blocks Manual injections B.
BioOptix™ 10 Appendix B Fluid Path Diagrams B.4 Pump Flush A Pump Flush should be done whenever the system will be idle for more than a few hours. (See sections 3.8 and 4.1.) The flush removes salts from the Buffer in lines to the Mode knob (Figure B-6). To Pump Purge Channel Mode Sample Buffer Pump Buffer A Buffer B Figure B-6 Pump flush B.5 Plumbing Diagram B-4 A complete plumbing diagram can be found on the following page.
BioOptix™ 10 Appendix B Fluid Path Diagrams Figure B-7 BioOptix 10 Plumbing Diagram B-5
BioOptix™ 10 Appendix B Fluid Path Diagrams B-6
BioOptix™ 10 Appendix C Replacement Parts Lists Replacement parts are called out in illustrations in this section. Reference the call-outs in the accompanying tables to determine the part number for the item. Replacement parts can be purchased by contacting Teledyne Isco’s Customer Service Department. Teledyne Isco, Inc. Customer Service Department P.O. Box 82531 Lincoln, NE 68501 USA Phone: (800) 228-4373 (402) 464-0231 FAX:(402) 465-3022 E-mail:info@isco.
BioOptix™ 10 Appendix C Replacement Parts Lists C-2
13BioOptix™ 10 Replacement Parts Lists 13BioOptix™ 10 Replacement Parts Lists C.
13BioOptix™ 10 Replacement Parts Lists C-4
13BioOptix™ 10 Replacement Parts Lists C-5
13BioOptix™ 10 Replacement Parts Lists C-6
13BioOptix™ 10 Replacement Parts Lists C.
13BioOptix™ 10 Replacement Parts Lists C-8
13BioOptix™ 10 Replacement Parts Lists C-9
13BioOptix™ 10 Replacement Parts Lists C-10
13BioOptix™ 10 Replacement Parts Lists C.
13BioOptix™ 10 Replacement Parts Lists C-12
13BioOptix™ 10 Replacement Parts Lists C-13
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Compliance Statements DECLARATION OF CONFORMITY Application of Council Directive: Manufacturer's Name: Manufacturer's Address: Standard of C on fo r m ity Equipment Type/Environment: Trade Name/Model No: Year of Issue: Standards to which Conformity is Declared: 89/336/EEC – The EMC Directive 73/23/EEC – The Low Voltage Directive Teledyne Isco, Inc. 4700 Superior, Lincoln, Nebraska 68504 USA Mailing Address: P.O.
Warranty Teledyne Isco One Year Limited Factory Service Warranty * Teledyne Isco warrants covered products against failure due to faulty parts or workmanship for a period of one year (365 days) from their shipping date, or from the date of installation by an authorized Teledyne Isco Service Engineer, as may be appropriate. During the warranty period, repairs, replacements, and labor shall be provided at no charge.
