Manual

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[ Care and Use ManUal ]

PoraPak

™

RXN Cartridges and Bulk Care & Use 1

Contents

I. Common ApplICAtIons for CleAnup

II. protoCols

III. produCt GuIdelInes

porApAk rXn CArtrIdGes

IntroduCtIon

Waters PoraPak

™

Rxn cartidges and bulk are a family of polymer-based

chromatography products for designed for cleanup of synthetic reactions.

PoraPak Rxn products are available in two chemistries:

• PoraPakRxnCX,astrongcation-exchangesorbent

• PoraPakRxnRP,areversed-phasesorbent.

PoraPak Rxn sorbents are based on copolymers that exhibit the

following properties:

• Hardmaterialthatdoesnotdevelopincreasing

back pressure with flow.

• Littleswellingorshrinkingacrossarangeof

solventsandpHextremes.

• Lowhydraulicresistanceenablesflowbygravity.

• pHextremetolerancewithoutdissolutionorhydrolysis,

both limitations of silica-based sorbents.

This combination of physical and chemical properties makes PoraPak Rxn

cartridges ideal for synthesis cleanup. The polymers characteristics and

particlesizemaintaingravity,pressure-orvacuum-assistedflow;even

when reaction mixtures contain precipitate that may contribute additional

resistance to flow. The sample will still pass through the cartridge. The

polymer used in PoraPak Rxn products is resistant to shrinking or swelling

in the organic solvents typically used in synthetic reactions.

Summary of content (5 pages)

PAGE 1
[ Care and Use Manual ] PoraPak RXN Cartridges Introduction Contents Waters PoraPak™ Rxn cartidges and bulk are a family of polymer-based I. Common Applications for Cleanup chromatography products for designed for cleanup of synthetic reactions. II. Protocols PoraPak Rxn products are available in two chemistries: • PoraPak Rxn CX, a strong cation-exchange sorbent III. Product Guidelines • PoraPak Rxn RP, a reversed-phase sorbent.
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[ Care and Use Manual ] I.
PAGE 3
[ Care and Use Manual ] II. Protocols Using PoraPak Rxn CX: Strong Cation-Ion Exchange Catch-and-Elute and Pass-Through Protocols Catch and Elute Pass Through Condition Step 100% methanol Condition with an organic solvent such as methanol. T his step is important to wet the sorbent. Condition Step 100% methanol Load Step Fraction collected contains acids, neutrals and reaction solvent Pour the reaction mixture into the cartridge. Let the reaction mixture flow by gravity.
PAGE 4
[ Care and Use Manual ] III. Product Guidelines The following tables should be used as guidelines for loading capacity, flow rates, and volumes of wash and elution solvents. These tables take into consideration the dimension of the device and the bed volumes suggest appropriate flow rates and volumes for the condition, wash and elute steps.
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[ Care and Use Manual ] Sales Offices: Austria and European Export (Central South Eastern Europe, CIS and Middle East) 431 877 18 07 Australia 2 9933 1777 Belgium 32 2 726 1000 Brazil 55 11 5094 3788 Canada 800 252 4752 China 8621 6495 6999 CIS/Russia +7 495 3367000 Czech Republic 42 02 617 11384 Denmark 45 46 59 8080 Finland +358 9 5659 6288 France (33) 1 30 48 72 00 Germany 49 6196 400600 Hong Kong 852 29 64 1800 Hungary 36 1 350 5086 India and India Subcontinent 91 80 2 837 1900 Ireland 353 1 448 1500