Technical data

2 Sample Preparation (3 µg DNA Samples)

Step 1. Shear the DNA

22 SureSelect

Target Enrichment System for Illumina Multiplexed Sequencing

Step 1. Shear the DNA

Make sure genomic DNA samples are of high quality with an OD 260/280 ratio ranging

from 1.8 to 2.0. Use the Qubit system to quantify genomic DNA before library preparation.

For each DNA sample to be sequenced, prepare 1 library.

1 Set up t

he Covaris E-series or S-series instrument.

a Check that the water in the Covaris tank is filled with fresh

deionized water to the appropriate fill line level according to the

manufacturer’s recommendations for the specific instrument model

and sample tube or plate in use.

b Check that the water covers the visible glass part of the tube.

c On the instrument control panel, push the Degas button. Degas the

instrument for least 30 minutes before use, or according to the

manufacturer’s recommendations.

d Set the chiller temperature to between 2°C to 5°C to ensure that the

temperature reading in the water bath displays 5°C.

e Optional. Supplement the circulated water chiller with ethylene

glycol to 20% volume to prevent freezing.

Refer to the Covaris instrument user guide for more details.

2 Put a Covaris microTube into the loading and unloading station.

Keep the cap on the tube.

You can use the 96 microTube plate (see Table 6 on page 17) for the DNA shearing step

when preparing multiple gDNA samples in the same experiment.

3 Use the Qubit dsDNA Assay to determine the concentration of your

gDNA sample.

Follow the instructions for the instrument.

4 Dilute 3 µg of high-quality gDNA with 1X Low TE Buffer in a 1.5-mL

LoBind tube to a total volume of 130 µL.

5 Use a tapered pipette tip to slowly transfer the 130-µL DNA sample

through the pre-split septa.

Be careful not to introduce a bubble into the bottom of the tube.

NOTE