Technical data

2 Sample Preparation (3 µg DNA Samples)
Step 10. Amplify the adaptor-ligated library
36 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
3 Run t
he program in Table 18 in a thermal cycler.
Different library preparations can produce slightly different results, based on varying DNA
quality
. In most cases, five cycles will produce an adequate yield for subsequent capture
without introducing bias or non-specific products. If yield is too low, or too high (where
non-specific high molecular weight products are observed), adjust the number of cycles
accordingly to amplify the remaining library template.
.
Table 18 Pre-Capture PCR Thermal Cycler Program
Segment Number of
Cycles
Temperature Time
1 1 98°C 2 minutes
2 4–6 98°C 30 seconds
65°C 30 seconds
72°C 1 minute
3 1 72°C 10 minutes
4 1 4°C Hold
NOTE