Technical data

4 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
What’s New in Version B.2
• Support for the OneSeq Capture Libraries (see Table 4 on
page 16 and Table 40 on page 73).
• Support for ClearSeq Capture Libraries, including
ClearSeq Comprehensive Cancer XT Libraries (see Table 4
on page 16).
What’s New in Version B.1
• Support for kits with either 8-bp indexes A01 to H12
(revised index configuration, typically received December
2014 or later) or 6-bp indexes 1 to 16 (original index
configuration, typically received before December 2014).
Kits with 8-bp indexes include indexing primers provided
in white-capped tubes (16 Reactions) or blue plate
(96 Reactions). For indexing protocol details, see page 70.
For kit content details see page 88. For nucleotide
sequences of the 8-bp indexes, see page 92.
Kits with 6-bp indexes include indexing primers provided
in clear-capped tubes. For indexing protocol details, see
page 74. For kit contents see page 93. For nucleotide
sequences of the 6-bp indexes, see page 96.
• Support for sample processing in 96-well plates or 8-well
strip tubes. See Table 6 on page 17 for recommended
thermal cycler and plasticware. Protocol modifications for
plate/strip tube sample processing are located throughout
this guide. Key modifications include reduction in wash
solution volumes to 200 µL including ethanol washes (for
example, see step 8 on page 24) and capture washes (for
example, see step 6 on page 67).
• Revision of hybridization setup instructions (see page 60
through page 65). Revisions do not affect the final
composition of the hybridization reaction, but format and
order of certain preparation steps have been modified.
• Removal of SureSelect Elution Buffer and Neutralization
Buffer from SureSelect Target Enrichment Box 1 (p/n
5190-8645 or 5190-8646; see Table 49 on page 90).