Technical data

Sample Preparation (200 ng DNA Samples) 3
Step 2. Assess quality (optional)
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 45
Step 2. Assess quality (optional)
This step is optional.
Quality assessment can be done with the 2100 Bioanalyzer instrument.
For analysis of 200 ng sheared DNA samples, Use a High Sensitivity
DNA chip and reagent kit. See the High Sensitivity DNA Kit Guide at
www.genomics.agilent.com for more information on doing this step.
1 Check that the 2100 Bioanalyzer electrodes have been cleaned as
instructed in the reagent kit guide.
2 Open the 2100 Expert Software (version B.02.02 or higher), turn on the
2100 Bioanalyzer and check communication.
3 Prepare the chip, samples and ladder as instructed in the reagent kit
guide, using 1 µL of each sample for the analysis.
4 Load the prepared chip into the 2100 Bioanalyzer and start the run
within five minutes after preparation.
5 Within the instrument context, choose the High Sensitivity DNA assay
from the drop down list.
6 Start the run. Enter sample names and comments in the Data and
Assay context.
7 Check that the electropherogram shows a DNA fragment size peak
between 120–150 bp. A sample electropherogram is shown in Figure 6.
Figure 6 Analysis of sheared DNA using a High Sensitivity DNA Bioanalyzer assay.