Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide Agilent Technologies
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In This Guide… This guide contains information to install and maintain the Multimode for 6210/6220 TOF. 1 Installation This chapter contains information to prepare the TOF instrument for the G1978A multimode source and to replace the existing source with the multimode source. 2 TOF Software and TOF Firmware Upgrade for Multimode This chapter contains information to update your software and firmware for the multimode source.
Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide
Contents 1 Installation 7 Installation 9 Step 1. Prepare to install 9 Step 2. Remove the TOF E-Tub Electronics 10 Step 3. Change the firmware chips on the Analyzer and Power Distribution Boards 11 Step 4. Install the three 10MΩ cables 14 Step 5. Install the HV control PCA and cables 16 Step 6. Attach the multimode source to the instrument 21 Step 7.
Contents Step 9. Run each of the methods created 57 Step 10. Calculate the response of Multimode Demo 65 Step 11.
Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide 1 Installation Installation 9 Step 1. Prepare to install 9 Step 2. Remove the TOF E-Tub Electronics 10 Step 3. Change the firmware chips on the Analyzer and Power Distribution Boards 11 Step 4. Install the three 10MΩ cables 14 Step 5. Install the HV control PCA and cables 16 Step 6. Attach the multimode source to the instrument 21 Step 7.
1 Installation APCI Corona HV cable +/- 2kv APCI High voltage Vaporizer Heater Cable 8 Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide
Installation 1 Installation Step 1. Prepare to install 1 Check that you have these parts: • Bundled LC/MSD Multimode ESI/APCI Source (p/n G1978A) • LC/MSD Multimode ESI/APCI Source (p/n G1978-65239) • LCMSD-TOF MM ESI/APCI Enablement Kit (p/n G1978-60251) • TOF Software A.02.02 or higher • TOF Multimode Installation Guide (p/n G1978-90007) 2 Check that the TOF instrument has serial numbers greater than US50600501 or US54100700. 3 Check that the TOF has Smart Card 4.
1 Installation 8 In the MS TOF tab, click Parameters and select Negative ion polarity. Click Save As to save the negative tune parameters as d:\Program Files\Agilent\ TOF Software\Tune\NegativeESI.prm. 9 Right-click on the TOF icon in the Status pane and click Vent. 10 After the TOF system has completed the vent process, turn off the front switch and the main breaker. 11 Prepare a static-free work surface to store the electronics assemblies that are removed during the upgrade procedure. Step 2.
Installation 1 Step 3. Change the firmware chips on the Analyzer and Power Distribution Boards The Main PLCC Firmware chip U129 must be changed on the Analyzer 3 PCA for source identification (p/n G1978-80067). Two chips on the power distribution board U6 (p/n G1978-80100) Programmed ROM MM LON and U18 Chip EPROM (p/n G1978-80200) will also be changed. 1 Locate the Firmware Upgrade Kit, MM (part number G1978-60156) in the upgrade kit parts box. 2 Locate the Analyzer board that was removed in “Step 2.
1 Installation Figure 2 Carefully remove the chip from the socket 5 In the Firmware Upgrade Kit, find the chip with the part number G1978-80067. 6 Find the correct orientation of the chip so that it fits into the socket. Press the chip into the socket. CAUTION Be sure that the chip is completely in the socket. Otherwise, when the board is energized the chip will be destroyed. 7 Locate the Power Distribution Board (PDB) that was removed in “Step 2. Remove the TOF E-Tub Electronics” on page 10.
Installation 1 U6 Socket U18 Socket Figure 3 Remove the U6 and U18 chips from their sockets 9 Locate the chip with the part number G1978-80200. Find the correct orientation of the chip so that it fits into the socket. Press the G1978-80200 chip into the U18 socket. CAUTION Be sure that the chip is completely in the socket. Otherwise, when the board is energized the chip will be destroyed. 10 Locate the chip with part number G1978-80100.
1 Installation Step 4. Install the three 10MΩ cables 1 While the instrument is vented and the analyzer board is out, install the cables in series with the output cable for the Vcap/Vchamber and APCI power supplies. The cables can be installed with the analyzer board installed. It is easier with the analyzer board out. Figure 4 Left: Two M-F 10 MΩ ballast MIDGI cables (p/n G1978-60806) and one M-F 10MΩ ballast SCID cable (p/n G1978-60805).
Installation 1 3 Connect the other M-F 10MΩ ballast MIDGI cable (p/n G1978-60806) in series with the VChamber power supply cable and plug it back into to VChamber power supply output. 4 Connect the M-F 10MΩ ballast SCID cable (p/n G1978-60805) in series with the Vcap cable then plug it back into to VCap output of the power supply. Figure 6 Cables tucked to the side of the power supplies 5 Install the analyzer board if it was not already installed.
1 Installation Step 5. Install the HV control PCA and cables 1 Attach the attach the RS-232 cable to the HV and control PCA power supply RS 232 connector. Figure 7 Attaching RS-232 Cable 2 Remove the cover from the HV module (p/n G1978-60050) Figure 8 Removing HV Module Cover 3 Attach the HV and control PCA to the tube. The instrument front cover, top cover, safety cover with magnet, and side panel access door should be off. 4 Remove the plastic cable clamp from the desolvation heater cable.
Installation 1 Plastic cable clamp Figure 9 Plastic cable clamp Plastic cable clamp removed Figure 10 Cable clamp removed and cable moved down 5 Attach the HV and control PCA power supply to the tube with the self-trapping screw supplied.
1 Installation Attach the HV and control PCA power supply to the tub Figure 11 Self tapping screw 6 Clamp the top cover of the HV and control PCA power supply with screws provided to the support bracket.
Installation 1 Serial connector on Smart card Figure 13 RS-232 cable connections 7 Connect the (RS-232) from HV module to the Serial connector on Smart card 4 interface. See Figure 13. 8 Connect the 15V dc power supply to the HV and control PCA. See Figure 14 and Figure 15. 9 Connect the other end of the 15V dc power supply into an 110V ac outlet using the power cord supplied with the 15V dc power supply. See See Figure 14 and Figure 15.
1 Installation 15V dc connection to HV Module Figure 14 RS 232 cable connections 15V dc connection to HV module 110 VAC Power cord 15V dc power supply Figure 15 20 Power cord and 15V dc supply Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide
Step 6. Attach the multimode source to the instrument 1 Remove the currently installed spray chamber (if applicable) from the spray chamber mount. Remove the shipping cover from the multimode source spray chamber. Figure 16 Multimode source 2 Install the multimode source on the spray chamber mount. See Figure 17.
1 Installation 3 Screw the End Cap Assembly into the holder for the end cap. See Figure 18. Note that the field shaping electrodes are in the 9 o'clock and 6 o'clock positions. 4 Loosen the end plate screws on each side to adjust the field shaping electrodes position. Field shaping electrode, 9 o’clock position Field shaping electrode, 6 o’clock position Figure 18 Multimode End Cap Assembly (p/n G1978-60060) 5 Connect the vaporizer heater, APCI high voltage, and HV and control PCA cables.
Installation 1 APCI Heater 15V dc connection ESI Charging Electrode power APCI High Voltage Figure 19 Cable connections Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide 23
1 Installation Step 7. Install the nebulizer, corona needle, and gas connections 1 Install the nebulizer on the multimode source spray chamber. Never use a nebulizer spacer on a multimode source. 2 Connect the 1/8-inch nebulizing gas tubing from the LC/MS instrument mainframe to the nebulizer gas fitting 3 Connect the LC/MS sample tubing to the nebulizer zero dead volume fitting. See Figure 20. 4 Connect the LC to the Inlet connection on the TOF instrument.
Installation 1 6 Switch Calibrant Bottle B to the Multimode Calibrant (p/n G1969-85020) for TOF. Figure 21 Calibrant For TOF (p/n G1969-85020) 7 Re-install all covers.
1 Installation Changing Sources To convert between the ESI, APCI or APPI and the multimode ion source Do this step if you want to change the source between ESI, APCI or APPI and the multimode source. To change to the multimode source for the first time, go to “Installation” on page 7 instead. 1 Change the Source setting to minimum for drying gas, nebulizer gas, vaporizer temp, and drying gas temp. 2 Disconnect the nebulizer gas tube from the current ion source.
Installation 1 Standard spray shield Capillary cap Figure 23 Standard spray shield 6 Remove the capillary cap. If needed, moisten a clean cloth with isopropyl alcohol and wipe the capillary cap See Figure 24. Capillary cap Figure 24 Capillary Cap 7 If needed, moisten a clean cloth with isopropyl alcohol and gently wipe the front of the capillary then reinstall capillary cap.
1 Installation \ Capillary Figure 25 Capillary 8 After cleaning the capillary and cap, place the capillary cap back on capillary. Install the new spray shield with field shaping electrodes. Figure 26 End Cap Assembly, Multimode (p/n G1978-60060) 9 Screw the multimode spray shield into the holder for the spray shield. See Figure 27. Note that the field shaping electrodes are in the 9 o'clock and 6 o'clock positions.
Installation 1 Field shaping electrode, 9 o’clock position Field shaping electrode, 6 o’clock position Figure 27 Multimode End Cap Assembly (p/n G1978-60060) 11 Remove any cables such as APCI vaporizer heater cable and APCI high voltage cable and any other cables such as RS 232 cable on APPI source. CAUTION When converting back to any of the sources other than the G1978A the HV module, disconnect the +15V DC power from the module.
1 30 Installation Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide
Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide 2 TOF Software and TOF Firmware Upgrade for Multimode Step 1. Upgrade the TOF Software 32 Step 2. Upgrade the TOF firmware 35 Step 3. Pump down the LC/MSD TOF and condition the high voltage electronics 36 This section describes upgrading the G3300AA TOF Software to Revision A.02.02 and upgrading the TOF firmware to 5.30 or higher for the multimode source.
2 TOF Software and TOF Firmware Upgrade for Multimode Step 1. Upgrade the TOF Software 1 Back up the following folders in the C:\Program Files\Agilent\TOF Software folder to a safe location. • DA methods • methods • worklists Methods and mass lists will be overwritten when the new software is installed. Also, if you have modified any Agilent supplied files, these files may be overwritten when the software is upgraded. Back up any modified Agilent files to a safe location.
TOF Software and TOF Firmware Upgrade for Multimode 2 17 Click Next after specifying the destination location for the installed software. It is recommended to use the default location. 18 Mark the Install BootP check box. SmartCard 4 uses a fixed IP address (192.168.254.12). Therefore the BootP service is not needed for the TOF instrument. However, the LAN card for the LC may be configured to use BootP. You can also configure the LC with a fixed IP addresses.
2 TOF Software and TOF Firmware Upgrade for Multimode 25 After reloading software, configure the logical instrument. The TOF and optionally the LC and ADC are configured into one logical instrument. This must be done before using the TOF Software system. a Click Start > Programs > Agilent > TOF Software > Tools > Instrument Configuration. b Click Add. c In the Add Devices window, click OK to accept the default connection value for the LC/MSD TOF.
TOF Software and TOF Firmware Upgrade for Multimode 2 Step 2. Upgrade the TOF firmware 1 Install the TOF Firmware Update tool. Use File Explorer and open the CD-ROM drive for the Installation CD. 2 Open the folder Support\TOF Firmware. 3 Double-click MSFirmwareUpdate_1.16.exe. The WinZip self-extractor dialog appears. Use the default installation path. 4 Click Unzip. 5 Click OK when you are informed that the files have been unzipped. 6 Click Close to complete the installation. 7 Double-click tofupdate.
2 TOF Software and TOF Firmware Upgrade for Multimode Step 3. Pump down the LC/MSD TOF and condition the high voltage electronics Before you try tuning the LC/MSD TOF, the high voltage electronics need to be conditioned. 1 Click Start > Programs > Agilent > TOF Software > Tools > TOF Diagnostics. 2 Click Connection > Connect. By default, the IP address 192.168.254.12 for the LC/MSD TOF appears. 3 Click OK. Once you are connected, the MS Status line will say Instrument Responding.
Agilent G1978A Multimode for 6210/6220 TOF Set-Up Guide 3 Installation Verification Step 1. Auto tune 38 Step 2. Set up method names and parameters 44 Step 3. Create MMCHECKTOF_EI_POS.m 45 Step 4. Create MMCHECKTOF_EI_NEG.m 47 Step 5. Create MMCHECKTOF_CI_POS.m 49 Step 6. Create MMCHECKTOF_CI_NEG.m 51 Step 7. Create MMCHECKTOF_MX_EI POS_CI POS.m 53 Step 8. Create MMCHECKTOF_MX_EI NEG_CI NEG.m 55 Step 9. Run each of the methods created 57 Step 10. Calculate the response of Multimode Demo 65 Step 11.
3 Installation Verification Step 1. Auto tune MassHunter TOF Acquisition for TOF A.02.02 Before the TOF system with multimode source can be used, the installation and verification tasks must be completed. Auto tune is done in mixed mode, with the TOF G1969-85000 ESI-L Low Concnetration Tuning Mix. 1 Click File > Open > Method. 2 Load the MMIautotune.m file. 3 Click the Tune tab and select Autotune. The instrument will generate a positive Autotune report for the multimode source. See Figure 28 on page 39.
Installation Verification Figure 28 3 Positive Autotune report for multimode source Agilent Multimode for 6210/6220 TOF Installation and Maintenance Guide 39
3 Installation Verification 4 Switch the polarity to negative and run autotune. The instrument will generate a negative Autotune report for the multimode source.
Installation Verification 3 5 Load the associated polarity switching method positive. Example: MMIautotunePSPos.m 6 Click the Tune tab and select Autotune. The instrument will generate a polarity switching positive report only for MMIautotunePSPos.m.
3 Installation Verification 7 Load the associated polarity switching method negative. Example: MMIautotunePSNeg.m. The instrument will generate a polarity switching negative report only for MMIautotunePSNeg.m. The instrument is ready for use with fast polarity switching tunes.
Installation Verification 3 For MassHunter Workstation Software - Acquisition for TOF B.01.03 • Run autotune with the G1969-85000 Tune Mix. There are no tune specific methods. • Tune the 6220 in 2GHz extended dynamic range for both positive and negative. • Tune the 6210 in Standard (3200 m/z) mode 1GHz.
3 Installation Verification Step 2. Set up method names and parameters 1 Create six methods from Default.m for the multimode ESI + APCI LC Demo Sample (p/n G1978-85000), using these method names: • MMCHECKTOF_EI_POS.m • MMCHECKTOF_EI_NEG.m • MMCHECKTOF_CI_POS.m • MMCHECKTOF_CI_NEG.m • MMCHECKTOF_MX_EI POS_CI POS.m • MMCHECKTOF_MX_EI NEG_CI NEG.m 2 Use these parameters for each method: Table 1 Parameter/Tab Value Column Cartridge Hardware, Rapid Resolution, (p/n 820555-901) SB-C18 Rapid Res 3.5um,2.
Installation Verification 3 Step 3. Create MMCHECKTOF_EI_POS.m The graphics in this topic differ slightly for MassHunter B.01.03 or higher. For B.01.03, access these tabs from the Acquisition view. • Set the parameters for MMCHECKTOF_EI_POS.
3 Installation Verification Ionization Mode MM-ES Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Off Solvents Solvent A 100.0 % (65%MeOH:35%H2O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar Spray Chamber [MSZones] 46 Gas Temp 350 °C Maximum 350 °C Vaporizer 200 °C Maximum 250 °C Drying Gas 5.0 L/min Maximum 13.
Installation Verification 3 Step 4. Create MMCHECKTOF_EI_NEG.m • Set the parameters for MMCHECKTOF_EI_NEG.
3 Installation Verification Ionization Mode MM-ES Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Off Solvents Solvent A 100.0 % (65%MeOH:35%H2O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar Spray Chamber [MSZones] 48 Gas Temp 350 °C Maximum 350 °C Vaporizer 200 °C Maximum 250 °C Drying Gas 5.0 L/min Maximum 13.
Installation Verification 3 Step 5. Create MMCHECKTOF_CI_POS.m • Set the parameters for MMCHECKTOF_CI_POS.
3 Installation Verification Ionization Mode MM-APCI Polarity Positive 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Off Solvents Solvent A 100.0 % (65%MeOH:35%H2O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar Spray Chamber [MSZones] 50 Gas Temp 350 °C Maximum 350 °C Vaporizer 200 °C Maximum 250 °C Drying Gas 5.0 L/min Maximum 13.
Step 6. Create MMCHECKTOF_CI_NEG.m • Set the parameters for MMCHECKTOF_CI_NEG.
3 Installation Verification Ionization Mode MM-APCI Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Off Solvents Solvent A 100.0 % (65%MeOH:35%H2O + 0.2%acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar Spray Chamber [MSZones] 52 Gas Temp 350 °C Maximum 350 °C Vaporizer 200 °C Maximum 250 °C Drying Gas 5.0 L/min Maximum 13.
Installation Verification 3 Step 7. Create MMCHECKTOF_MX_EI POS_CI POS.m • Set the parameters for MMCHECKTOF_MX_EI POS_CI POS.m.
3 Installation Verification Ionization Mode MM-ES+APCI Polarity Positive 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Off Solvents Solvent A 100.0 % (65%MeOH:35%H2O + 0.2% acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar Spray Chamber [MSZones] 54 Gas Temp 350 °C Maximum 350 °C Vaporizer 200 °C Maximum 250 °C Drying Gas 5.0 L/min Maximum 13.
Installation Verification 3 Step 8. Create MMCHECKTOF_MX_EI NEG_CI NEG.m • Set the parameters for MMCHECKTOF_MX_EI NEG_CI NEG.
3 Installation Verification Ionization Mode MM-ES+APCI Polarity Negative 1100 Binary Pump 1 Control Column flow 0.400 mL/min Stop Time No Limit Post Time Off Solvents Solvent A 100.0 % (65%MeOH:35%H2O + 0.2% acetic acid)) Solvent B 0.0 % Pressure Limits Minimum Pressure 0 bar Maximum Pressure 400 bar Spray Chamber [MSZones] 56 Gas Temp 350 °C Maximum 350 °C Vaporizer 200 °C Maximum 250 °C Drying Gas 5.0 L/min Maximum 13.
Installation Verification 3 Step 9. Run each of the methods created 1 Run each of the methods that you just created. The real time plot below shows the six runs. ESI +APCI NEG 1-Hexanesulfonic acid & 9-Phenanthrol ESI POS Crystal Violet ESI NEG 1-Hexanesulfonic acid APCI POS Carbazole APCI NEG 9-Phenanthrol ESI +APCI POS Crystal Violet & Carbazole 2 View the data from Analyst for MM_ESI_pos.wif. Exstract Ion 372- 372.4. Record peak height Example: 91,000.
3 Installation Verification 3 View the data in the data analysis program for MM_ESI_Neg. Extract Ion 165-165.4. Record the peak height Example 97,000.
Installation Verification 3 4 View the data in the data analys program for MM_APCI_POS. Extract Ion 168-168.4. Record the peak height. Example 140,000.
3 Installation Verification 5 View the data in the data analysis program for MM_APCI_NEG. Extract Ion 193-193.4. Record the peak height. Example 640,000.
Installation Verification 3 6 View the data in the data analysis program for MM_ ESI_APCI_POS. Extract Ion 372-372.4. Record the peak height. Example: 57,000.
3 Installation Verification 7 View the data in the data analysis program for MM_ ESI_APCI_POS. Extract Ion 168-168.4. Record the peak height. Example: 34,000.
Installation Verification 3 8 View the data in the data analysis program for MM_ ESI_APCI_NEG. Extract Ion 165-165.4. Record the peak height. Example: 110,000.
3 Installation Verification 9 View the data in the data analysis program for MM_ ESI_APCI_NEG. Extract Ion 193-193.4. Record the peak height. Example: 400,000.
Installation Verification 3 Step 10. Calculate the response of Multimode Demo 1 Manually fill in the values in the Multimode Ion Source report. The values in the example report below have been manually entered from the data collected in the runs from the previous steps. This is an example of how to enter the values from the instrument being installed and verified. The blank report is on the next page for installed instruments data. 2 Run all methods and get the peak heights.
3 Installation Verification Step 11. Fill out Multimode Report for calculation of peak heights • Use the graphic below to fill out the multimode report for calculation of peak heights.
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