Technical data

Understanding Your Agilent ChemStation 125

Quantification

ISTD Calculation

ISTD Calculation of Calibrated Peaks

The equations used to calculate the actual amount of a calibrated component x

for a single-level calibration are:

where:

is the response factor for compound x;

The actual amount (Actual Amt) of ISTD is the value that was entered in the

Calibration Settings dialog box or the Sample Info dialog box for the internal

standard added to the unknown sample;

M is the multiplier.

D is the dilution factor.

If the ISTD% report type is selected, the following equation is used to calculate

the relative (%) amount of component x:

ISTD Calculation of Uncalibrated Peaks

There are two ways to define the response factor which is used to calculate the

amount for unidentified peaks.

1 Use the fixed response factor set in the With Rsp Factor box of the

Calibration Settings dialog box. You can choose to correct the fixed

response factor by specifying an ISTD correction.