Technical data
186 Understanding Your Agilent ChemStation
8 Automation
Cyclic Multiple-Level Calibration Sequences
Cyclic Multiple-Level Calibration Sequences 
This type of sequence uses different calibration samples to recalibrate a 
multiple-level calibrated method.
The following example describes a two-method sequence comprising method A 
and method B to analyze two groups of samples. Both methods are 
multiple-level calibration methods that will recalibrate automatically at 
defined intervals.
For each method the Sequence Table has three entries: 
• Two calibration levels: 
• Sequence lines 1 and 2 in method A.
• Sequence lines 8 and 9 in method B.
• Five entries for the samples: 
• Sequence line 3 through 7 in method A.
• Sequence line 10 through 14 for method B.
The calibrations are specified at regular intervals by the recalibration interval 
entry in the Sequence Recalibration Table. 
• Method A will recalibrate after every two samples.
• Method B will recalibrate after every three samples.
The Sequence Table below is truncated to simplify the example.
Table 1 6 Sequence Table for Method A and Method B
Line Vial Method 
Name
Inj/Vial Sample 
Type
Cal 
Level
Update RF Update RT Interval
1 1 Method A 1 Calibration 1 Average No update 2
2 2 Method A 1 Calibration 2 Average No update 2
310 Method A 1
411 Method A 1
512 Method A 1










