User manual

18 Agilent 1260 Infinity High Performance Autosampler User Manual
1 Introduction
Autosampler Principle
Inject-and-Run The final step is the inject- and- run step. The six- port valve is switched to
the main- pass position, and directs the flow back through the sample
loop, which now contains a certain amount of sample. The solvent flow
transports the sample onto the column, and separation begins. This is the
beginning of a run within an analysis. In this stage, all major
performance- influencing hardware is flushed internally by the solvent
flow. For standard applications no additional flushing procedure is
required.
Figure 5 Inject and Run