Troubleshooting guide
Lab Exercise: Sequence Review and Reprocessing 
Reprocess with Sequence Folder Method 
114 
7)  Now, take a look at the calibration curve. Select the Calibration menu and 
then Calibration Table. 
8)  Click next to the 4
th
 level row to highlight the line and press the Delete 
button. 
9)  Remove all level 4 rows from the table. 
10)  Notice that the Correlation is now improved. 
11)  Save the sequence folder method. 
Always check to make certain you are in the correct directory path. Fill in the 
change history stating the the retention time window was updated and the 4
th
 level 
calibrator was removed. 
12)  During the original sequence acquisition, there was quite a large sequence 
summary report as well as individual data files printed. We do not want to 
print that much information here. Therefore, we will change the sequence 
output. This is a sequence, .S file, parameter. The tools shown will allow 
you to make changes to this file without going to the Method and Run 
Control view. 










