Troubleshooting guide
Lab Exercise: Acquisition Methods 
Preparing the HPLC 
20 
Preparing the HPLC 
1)  Enter the Method and Run Control view of the Agilent Online ChemStation. 
2)  From the View menu, select Instrument Actuals, the System Diagram and 
Sampling Diagram. If a check mark exists beside the item, it is already on. 
3)  Load the default method, DEF_LC.M as a starting point for method creation 
(Method, New Method) or double-click on DEF_LC.M in the ChemStation 
Explorer. 
4)  Under the Instrument menu, select More DAD, Control. Turn on the UV 
and Vis lamps. OK the window. Remember that you may also turn the lamp 
on from the system diagram GUI. 










