Troubleshooting guide

Lab Exercise: Acquisition Methods
Priming the Agilent 1100/1200 Solvent Delivery System
21
Priming the Agilent 1100/1200 Solvent Delivery
System
You should prime the pump when you change mobile phases, when the
instrument has been sitting without flow for long periods of time, when you have
performed maintenance on the HPLC, or when you experience periodic
perturbations in the baseline.
1) If you are equipped with a vacuum degasser, make certain the degasser is on.
2) Ensure that the outlet tube is connected from the purge valve to the waste
container. Open the purge valve located on the pump module by turning the
knob counter clockwise several turns.
3) To prime the solvent delivery system, you should pump 100% at 5 mL/min
from each channel for several minutes. To accomplish this task, select the
following menu items: Instrument, Set up Pump...; or click on the pump in
the system diagram (shown below), then Set up Pump....
4) Type in a Flow of 5.000 mL/min and %A of 100. OK the window.
5) To turn the solvent delivery system on, you may use the menu items:
Instrument, More Pump, Control; or, access the tool shown in the diagram
below. Do this now.
Click here