Troubleshooting guide

Lab Exercise: Acquisition Methods
Priming the Agilent 1100/1200 Solvent Delivery System
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6) Wait until a steady stream of solvent comes out of the purge valve waste tube.
7) Repeat steps 3 through 6 for the other channels of the pump. Change to the
composition for your next run, %B=70.
8) Turn off the pump.
9) Close the purge valve. Set the required composition and flow rate for your
next application. In this case: Flow 1.5 mL/min and %B 70.
10) Turn on the pump and allow the column to equilibrate.
Note: Always keep HPLC grade solvent in all pump channels to prevent valve
damage even when the channel is not being used. For a reversed phase system,
place water with 20% methanol in the unused channels.