Troubleshooting guide

Lab Exercise: Acquisition Methods

Practice Entering Gradients

1) Select the Insert button from the Time Table group. Program a linear

gradient to 100%B over 4.0 minutes. In the Time column, type 4. In the %B

column type 100.

2) Go to the Display dropbox. Select Solvents.

3) Return to the Timetable display. Click on the 1 next to the timetable line,

then cut. Unfortunately, you do not need a gradient for this analysis, OK the

Pump Parameters window.

Back to the Acquisition Method

1) Select a Standard Injection with a 5 µl injection volume. Select More... to

view all injection options.

Question:

What is the slowest draw speed? (hint: type in 1µl)

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When would you reduce the draw speed?

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2) OK the window.

3) Fill in the DAD Signals as below. OK the window.

Note: If a VWD detector is being utilized, select the same wavelength as signal