Troubleshooting guide

Lab Exercise: Integration

Integrate Using the Events Table

Now, you will set up integration events for the Integration.d chromatogram using

the approach discussed in lecture.

1) Click on the Integration tool, then the Edit Integration Events tool.

2) Using the signal drop box, select DAD1B Specific.

3) Set the Slope Sensitivity to 50.

4) Estimate the peak width from the initial integration. Use the smallest peak

width from a real chromatographic peak, not noise.

5) Set the initial height and area reject to zero. Set the Shoulders to OFF.

6) Set the Tail Peak Skim Height Ratio to 3, the Front Peak Skim Height Ratio

to 6, and the Skim Valley Ratio to 20.

7) Set the Baseline Correction mode to Advanced with the Tangent Skim Mode

set as New Exponential.

8) Integrate using the integration tool and view the results.

9) Print the chromatogram and notice any differences in integration.