Troubleshooting guide

Lab Exercise: Quantification

Integration

In this section you will optimize the integration events for the analysis and save

them to the method.

1) Go into the Data Analysis view.

2) From the View menu, select Preferences.

3) Select the Signal Options tab. Make certain that integrate after load is the

only box checked.

4) Find your data analysis directory in the ChemStation Explorer. Double-click

on the Single Runs to place the files in the Navigation Table. Find the data

file, Low.d, and load all signals. The data file is initially integrated using the

default integration events. Make certain both signals A and B are present.

5) After loading, go to the Integration menu and select Integration Events or

select the Select Integration Task tool, then the Edit/Set Integration Events

Table tool.

6) Select DAD Default for the Events Table and insert the following integration

events:

Tangent Skim Mode: New Exponential

Tail Peak Skim Height Ratio: 3

Front Peak Skim Height Ratio: 6

Skim Valley Ratio: 20

Baseline Correction: Advanced

Peak to Valley Ratio: 500

Slope Sensitivity: 50

Peak Width: Set to the narrowest real chromatographic peak

Area Reject: 0

Height Reject: 1

Shoulders Off

7) Reintergrate the chromatogram using the new events. Either select the

Integration menu then Integrate, or the Integrate tool.

8) Examine the current integration. Change settings appropriately. Remove

undesirable peaks (there should be four main peaks) using either the area

reject or timed events.