User`s guide

Contents ▲ 166 ▼ Index
Overview of Flow Cytometric Assays
The cell characteristic to be measured requires not only specific dyes. Several
measurement parameters to control the measurement and the data acquisition
parameters also have to be specified. These so-called “setpoints” are stored in assay
files (.xsy) and are read by the 2100 expert software before it starts the measurement.
2100 expert supports the following assays based on flow cytometry:
Predefined assays
• Apoptosis
• Apoptosis – fast protocol
For reduced background this assay has an increased threshold and uses blue events
only for peak detection.
• Antibody Staining
• On-chip Antibody Staining
For reduced background this assay has an increased threshold in the blue signal.
• GFP
• On-chip GFP
This assay allows a rapid and accurate detection of green fluorescent protein
expression.
• Blue to red
This assay is for applications that apply a blue reference dye and analyze red
fluorescent cells within a blue population.