User`s guide

Contents ▲ 167 ▼ Index
• Red to blue
This assay is for applications that apply a red reference dye and analyze blue
fluorescent cells within a red population.
• Checkout Beads
Red checkout beads are loaded into the wells 1, 3 and 5 and blue checkout beads into
the wells 2, 4 and 6. Markers are set according to expected fluorescence levels of the
red and blue beads. The Checkout Beads assay has the properties of a generic assay
(see below).
• siRNA Transfection Viability
Transfection Viability analysis as described in the Application Note: siRNA transfection
optimization with the Agilent 2100 bioanalyzer (Agilent publication number:
5988-9782EN). This assay enables the automatic calculation of transfection efficiency
(TE) in histogram view and viability in transfected cells (ViT) in dot plot view. Required
gating directions and regions are provided as example, but can be adjusted. Final
Transfection Viability (TV) can be calculated by multiplication TE and ViT values derived
from histograms and dot plots.
The settings of predefined assays are optimized to measure the appropriate cell
characteristics. For evaluation, it is only necessary to adjust the markers in histograms or
regions in dot plots.
Predefined assays contain all necessary markers and regions for evaluation. The gating
direction for histograms is given (for details on the gating direction, refer to “Gating” on
page 201). The markers in all samples are connected (changing a marker changes the
corresponding markers in all samples).