User`s guide

Contents ▲ 178 ▼ Index
Essential Measurement Practices (Flow Cytometric Assays)
• Handle and store all reagents according to the instructions given in the Reagent Kit
Guides.
• Avoid sources of dust or other contaminants. Foreign matter in reagents and samples
or in the wells of the chip will interfere with assay results.
• Store all reagent and reagent mixes in the dark and refrigerated at 4 °C when not in use.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Protect focusing dye from light. The dye decomposes when exposed to light.
• Use appropriate pipette tips. For each pipetting step use a fresh, new pipette tip.
• Always insert the pipette tip to the bottom of the well when dispensing the liquid.
Placing the pipette at the edge of the well may lead to poor results due to the formation
of a bubble on the bottom of the well.
• For chip preparation, use inverse pipetting.
• When filling the pipette tip, push slightly over the first resistance. Empty the pipette tip
only to the first resistance. This procedure avoids the introduction of bubbles and
ensures pipetting the right volume.
• Never leave any wells empty, or the pressure adapter may become clogged. Pipette
10 µl of cell buffer or sample replicate in any empty sample well.