User`s guide
Contents ▲ 232 ▼ Index
When using the calcein marker in the blue histogram for gating, only living cells are 
considered for building the histogram of the red dye. High red fluorescence values 
indicate living cells with bound antibodies, low red fluorescence values living cells 
without bound antibodies. See the following example.
The values are displayed in the result table below the red histogram:
Low fluorescence values 
indicate low binding of 
labeled antibodies
High fluorescence 
values indicate binding 
of labeled antibodies
Percentage of all cells with 
high red fluorescence 
selected by the red marker
Percentage of the living cells
Amount of living cells with high red fluorescence in relation to the amount of living cells










